匍匐翦股颖和狗牙根原生质体融合研究  

Study on Protoplast Fusion of Creeping Bentgrass and Bermuda Grass

作　　者：梁雪莲[1] 陈平[1] 佘伟标[1] 黄黉[1] 

机构地区：[1]仲恺农业技术学院生命科学院,广东广州510225

出　　处：《中国草地学报》2009年第4期63-68,共6页Chinese Journal of Grassland

基　　金：广东省科技攻关项目(2005B20901018);广州市科技攻关重点项目(2005Z2-E0201);广东省良种培育与引进项目(粤财农[2004]295号);仲恺农学院大学生创新基金项目(200508B)

摘　　要：以匍匐翦股颖克罗米和狗牙根新农1号为材料,通过愈伤诱导、继代和悬浮培养,进而细胞融合来探讨草坪草品种改良的新方法。结果表明:(1)匍匐翦股颖和狗牙根原生质体融合时酶解液的最佳组合为:1.5%纤维素酶、0.1%果胶酶、0.7M甘露醇,10h酶解时间,且酶解前应用高渗溶液预处理效果更佳;(2)细胞融合时,原生质体密度2×105个/mL,融合剂由30%的PEG4000、15mmol/L CaCl2.2H2O0、.5mmol/L KH2PO4.H2O、0.5mol/L甘露醇组成,并调pH至7.5效果较好;(3)匍匐翦股颖和狗牙根细胞PEG融合率约为5%,以30min融合时间效果较好,产生的多核融合体少,杂种细胞多呈条形,具有较强的活力。After several generations of successive transferation and suspension culture, the best sus- pended cell materials of Creeping Bentgrass and Bermuda grass were selected. Their cell wall were removed and carried on the protoplast fusion. The objective of this research was to find a new method to improve turf quality. The results indicated that： （1） The optimal enzymolysis solution involved 1.5% cellulases, 0.1% pectolase, and 0.7M mannitol; and 10 hours was the best enzymolysis time. In addition, pretreatment.of the protoplast in sugar solution before the enzymolysis had better effect. （2） The fusion agents included 30% PEG4000, 15mmol/L CaCl2 · 2H2O, 0.5mmol/L KH2PO4 · H2O and 0.5mol/L mannitol, and pH 7.5 . Before fusion, adjusted the protoplast volume density to 2×10^5/mL. （3） The cell fusion rates of the two turf materials was 5%, 30min of fusion time would be better to reduce coenocytia, the hybrid cells seemed to be monstrous but with high vitality.

关 键 词：匍匐翦股颖 狗牙根 原生质体融合 

分 类 号：S688.4[农业科学—观赏园艺] Q813.2[农业科学—园艺学]