黄芪多糖对哮喘大鼠气道炎症及IL-3表达的影响  

Effects of Astragalus polysaccharides on airway inflammation and interleukin-3 expression in asthmatic rats

作　　者：江茵[1] 黎东明[2] 陈敏[2] 

机构地区：[1]广东医学院诊断学与内科学教研室,广东湛江524001 [2]广东医学院附属医院呼吸疾病研究所,广东湛江524001

出　　处：《中国现代医学杂志》2009年第12期1779-1781,共3页China Journal of Modern Medicine

摘　　要：目的通过观察黄芪多糖对哮喘大鼠肺泡灌洗液细胞总数及嗜酸性粒细胞(Eos)计数、白细胞介素-3(IL-3)表达的影响,探讨黄芪多糖对哮喘的治疗作用及其可能机制。方法36只雄性SD大鼠随机分为正常对照组(A组)、哮喘模型组(B组)、黄芪多糖治疗组(C组)各12只,采用卵蛋白(OVA)致敏大鼠哮喘模型,观察3组动物哮喘发作症状、肺泡灌洗液总细胞数及嗜酸性粒细胞计数情况及原位杂交方法检测肺组织中IL-3在肺组织的表达变化。结果B组大鼠哮喘发作症状明显重于C组,A组无症状。B组肺泡灌洗液总细胞数及嗜酸性粒细胞计数均明显高于其余两组,C组明显少于B组,A组最少。肺组织中IL-3表达A组微弱表达,C组较强,B组表达最强。结论黄芪多糖可抑制哮喘大鼠的气道炎症,其作用机制可能是通过抑制IL-3的表达来实现。[Objective] To observe the effect of astragalus polysaccharides on interleukin -3 expression and airway inflammation in asthmatic rats. [Methods] 36 male Sprague-Dawley rats were randomly divided into normal control group （A group）, model group （B group）, curcumine treatment group （C group）, 12 rats in each group respectively. Rats were sensitized and challenged continually with ovalbumin （OVA） for the purpose of induction of asthma in rats. To observe asthmatic attack and airway inflammation in asthmatic rats. Interleukin -3 expression in rat pulmonary tissues was detected by using in situ hybridization method. [Results] Asthmatic attack obviously in B group, gently in C group, absence of symptoms in A group. The total cell amount in bronchoalveolar lavage fliud and eosinophil were obviously higher in B group than C group and A group. The expression of interleukin -3 mRNA in C group was significantly higher than that in A group and it was lower than that in B group. [Conclusion] The effect of astragalus pelysaccharides on airway inflammation in asthmatic rats may be correlated with its inhibition on over expression of interleukin -3 in lung tissue.

关 键 词：黄芪多糖 哮喘 IL-3 气道炎症 

分 类 号：R562.25[医药卫生—呼吸系统] R-332[医药卫生—内科学]