阜平大枣无芽茎段再生不定芽的初步研究  

Preliminary Study on Adventitious Bud Regeneration from in Vitro Non-bud Stem of Ziziphus jujuba Mill. ‘Fupingdazao’

作　　者：周小丽[1] 彭建营[1] 段红喜[2] 

机构地区：[1]河北农业大学园艺学院,河北保定071001 [2]中国地质大学长城学院,河北保定071000

出　　处：《中国农学通报》2009年第13期150-153,共4页Chinese Agricultural Science Bulletin

基　　金：河北省自然科学基金项目(C2007000448);河北农业大学科技将帅计划资助

摘　　要：以MS为基本培养基,幼嫩的阜平大枣无芽茎段为试材,研究了灭菌时间、暗培养、以及植物生长调节剂对无芽茎段诱导不定芽再生的影响。结果表明:无芽茎段以灭菌8～10min为宜,暗培养21天最佳,在MS基本培养基上附加6-BA1.0mg/L,IBA0.1mg/L再生率和平均出芽数显著高于其它处理,分别为85.42%和3.23,而且不定芽经培养后生长成枣头一次枝。The factors affecting adventitious bud regeneration from in vitro non-bud stem of Ziziphus jujuba Mill.‘Fupingdazao’ in MS media were studied, which included sterilization time, dark incubation time and plant growth regulator. The results showed that 8-10 minutes of sterilization time for young non-bud stem was suitable; 21 days of dark culture was optimal ;The best combination of plant growth regulators was confirmed on MS＋6-BA 1.0mg/L＋ IBA 0.1mg/L, on which regeneration rate and Adventitious buds per stem were 85.42% and 3.23 respectively, they were significantly higher than other treatment, and the adventitious buds could become developmental branch.

关 键 词：阜平大枣 无芽茎段 离体培养 

分 类 号：S665.1[农业科学—果树学]