二甲酚橙显色体系测定明胶中的钙含量  

Determination of Content of Calcium in Gelatin by Xylenol Orange

作　　者：于大勇[1] 黄越洋[1] 魏文武[1] 

机构地区：[1]辽宁石油化工大学石油化工学院应用化学系,辽宁抚顺113001

出　　处：《现代科学仪器》2009年第3期99-101,共3页Modern Scientific Instruments

摘　　要：以二甲酚橙—十六烷基三甲基溴化胺—无水乙醇络合显色体系测定了明胶中的钙。考查了测定条件。络合物最大吸收波长为560nm,摩尔吸光系数ε=1.0672×10~5 L·mol^(-1),相关系数r=0.9997,Ca^(2+)质量浓度在0～12.5μg范围内服从郎伯—比尔定律。合成样品其平均回收率为102.20%,实际样品明胶测定结果含钙为9.30μg·g^(-1),其相对标准偏差为0.37%。加标回收率平均为99.60%,其相对标准偏差为0.65%。利用本方法测定明胶中的钙含量,选择性高,准确度较好,方法简单,结果满意。The calcium was determined by xylenol orange-CTMAB-absolute ethyl alcohol complexation color in the gelatin. The test conditions were examined. We got that the best absorption of complexation is 560nm, the molar absorptivity is ε=1.0672×10^5 L·mol^-1, the correlation coefficient is r=0.9997, the mass concentration of Ca2~ is order to longbow-bill law in the 0-- 12.5 lag. The average recovery rate of synthetics is 102.20%, and the result of determination showed that it had 9.30μg.g^-1 calcium in the gelatin. The relative standard deviation was 0.37%. The recovery was 99.60%. The relative standard deviation was 0.65%. The calcium in the gelatin was determined by this measurement, the result showed that it has good selectivity and the better accuracy. It is the easy measurement which has a satisfactory result.

关 键 词：二甲酚橙 明胶 钙 

分 类 号：TS251.9[轻工技术与工程—农产品加工及贮藏工程]