小檗碱对四氯化碳致小鼠急性肝损伤的保护作用研究  

Protective Effect of Berberine on CCl_4-induced Acute Liver Injury in Mice

作　　者：李大庆[1] 吴明均[1] 

机构地区：[1]遵义医学院附属口腔医院药剂科,遵义市563000

出　　处：《中国药房》2009年第21期1618-1619,共2页China Pharmacy

摘　　要：目的:研究小檗碱对四氯化碳(CCl4)致小鼠急性肝损伤的保护作用。方法:选用昆明种小鼠,均分为6组,即正常、模型、联苯双酯和小檗碱高、中、低剂量(40、20、10mg.kg-1)组。连续灌胃给药5d后腹腔注射0.1%CCl4(10mL.kg-1)致小鼠急性肝损伤,测定小鼠血清中丙氨酸氨基转移酶(ALT)、天冬氨酸氨基转移酶(AST)活性,肝匀浆中超氧化物歧化酶(SOD)活性及丙二醛(MDA)含量。结果:与模型组比较,小檗碱各剂量组均能显著降低小鼠血清ALT和AST活性(P<0.05或P<0.01),升高小鼠肝脏SOD活性(P<0.05或P<0.01),降低肝脏MDA含量(P<0.05或P<0.01)。结论:小檗碱对CCl4所致的小鼠急性肝脏损伤有保护作用,其机制与提高小鼠的抗氧化能力有关。OBJECTIVE： To investigate the protective effect of berberine on CCl4- induced acute liver injury in mice. METHODS： Kunming mice were included and equally divided into 6 groups： normal group, model group, berberine groups （40,20,10 mg · kg ^-1） .After intragastric administration of corresponding drugs for 5 consecutive days, the mice were intraperitoneally injected with CCl4（10 mL · kg^- 1） to establish liver injury mice model. Alanine transaminase （ALT） and aspartate transaminase fAST） in serum, activities of superoxidase （SOD） and malondialdehyde （MDA） content in liver homogenate were measured. RESULTS： In Berberine-treated groups compared the model group, serum ALT level and AST activity decreased significantly（ P 〈 0.05 or P 〈 0.01）, SOD activity ehanced （ P 〈 0.05 or P 〈 0.01） while MDA content in hepatic tissue decreased （P 〈 0.05 or P 〈 0.01） .CONCLUSION： Berberine has protective effect on rats against CCl4-induced acute liver injury, and the mechanism may be related to the enhanced anti- oxidation ability of mice.

关 键 词：小檗碱 肝损伤 丙氨酸氨基转移酶 天冬氨酸氨基转移酶 超氧化物歧化酶 丙二醛 

分 类 号：R285.15[医药卫生—中药学] R975[医药卫生—中医学]