微乳TLC法分离鉴定肠激安方中多种有效成分  

Microemulsion Thin-layer Chromatography in the Separation and Identification of Various Effective Compositions in Changji'an Prescription

作　　者：唐洪梅[1] 闫雪[2] 李得堂[2] 

机构地区：[1]广州中医药大学第一附属医院,广州市510405 [2]广州中医药大学,广州市510405

出　　处：《中国药房》2009年第21期1633-1635,共3页China Pharmacy

基　　金：广东省医院药学研究基金(2008A016);广州市科技计划项(2005J1-C0201)

摘　　要：目的:建立以微乳薄层色谱法分离鉴定肠激安方中多种有效成分的方法。方法:以5种配比的十二烷基硫酸钠-正丁醇-正庚烷-水微乳系统为展开剂,聚酰胺薄膜为固定相,检测肠激安方中多种有效成分,并重点考察微乳液含水量、温度等因素对分离效果的影响。结果:以含水量75%的微乳液为展开剂可检出方中黄连;以含水量75%的微乳液-甲酸(9.0∶1.0)为展开剂可同时鉴定出方中延胡索、防风、绿原酸、甘草。结论:本方法简便、环保、灵敏、结果理想,可用于分离鉴定中成药中多种有效成分。OBJECTIVE： To establish the microemulsion TLC for the separation and identification of various effective compositions in Changji＇an prescription. METHODS： The compositions in Changji＇ an prescription were identified on polyamide film（fixed phase） with five distribution ratios of sodium lauryl sulphate- n- butanol- skellysolve C- H2O mieroemulsions as developing agents. The effects of factors such as the water content of the microemulsion and the temperature on the separation efficacy were studied. RESULTS： With microemulsion（75% water content） as developing agent, Rhizoma Coptidis in Changji＇an prescription was detected; with microemulsion（75% water content） -formic acid （9.0 ： 1.0） as developing agent, Corydalis yanhusuo, Saposhnikovia divaricata, chlorogenic acid and Radix et Rhizoma Glycyrrhizae in Changji＇an prescription were detected. CONCLUSION： Microemulsion TLC is simple, environmental - friendly, sensitive with ideal separation efficacy, and it can be used for the separation and identification of various compositions in Chinese patent medicines.

关 键 词：微乳 薄层色谱法 肠激安方 分离 鉴定 

分 类 号：R283[医药卫生—中药学] R927.1[医药卫生—中医学]