松辽黑猪×野猪杂交猪H-FABP mRNA基因的组织分布研究  

Distribution of H-FABP mRNA in Hybrids of Songliao Black Pig×Sus scrofa

作　　者：秦莹[1] 高妍[1,2] 张永宏[1] 刘同欣[1] 马倩[1] 朱银莉[1] 孙博兴[1] 赵志辉[1] 张嘉保[2] 

机构地区：[1]吉林大学畜牧兽医学院,吉林长春130062 [2]吉林大学实验动物中心,吉林长春130062

出　　处：《安徽农业科学》2009年第23期10902-10903,11043,共3页Journal of Anhui Agricultural Sciences

基　　金：国家高技术研究发展计划(2008AA10Z136);吉林省科技厅项目(20070567)资助

摘　　要：[目的]研究松辽黑猪×野猪杂交猪H-FABP mRNA基因的组织分布,为探明该基因的生理功能提供依据。[方法]采用RT-PCR技术获得松野杂交猪的cDNA,经PCR扩增后对目的片段进行电泳鉴定和测序,检测H-FABP mRNA在松辽黑猪×野猪杂交猪组织中的分布情况。[结果]在选取的皮下脂肪、腹脂、乳腺、背最长肌、臂三头肌、心脏、肝脏、脾脏、肺脏、肾脏、回肠、十二指肠共12种组织中,H-FABP mRNA均有表达。[结论]H-FABP基因在组织中广泛分布说明了其功能的重要性及多重性。[ Objective ] This study was to investigate the distribution of H-FABP mRNA in hybrids of Songliao black pig × Sus scrofa, so as to provide references for revealing the physiological functions of this gene. [ Method ] With the mRNA from different tissues as template, RT-PCR amplification was carried out for cloning cDNA of hybrid pig, which was then used for PCR reaction using specific primers; the amplification products were separated by gel electrophoresis and analyzed by sequencing for detecting the distribution pattern of H-FABP mRNA in hybrids of Songliao black pig x Sus scrofa. [ Result ] H-FABP expressed in all the twelve tissues including subcutaneous fat, abdominal fat, mammary gland, longissimus dorsi muscle, dorsal deltoid muscle, heart, liver, spleen, lung, kidney, ileum and duodenum. [Conclusion] The wide distribution of H-FABP gene suggests that its functions are important and multiple.

关 键 词：松辽黑猪×野猪杂交猪 H-FABP MRNA 组织分布 

分 类 号：S828[农业科学—畜牧学]