利用Asia1型口蹄疫病毒VP1蛋白的单克隆抗体建立单抗竞争ELISA方法  

Establishment of Monoclonal Antibody Competitive ELISA Using Monoclonal Antibody Against VP1 Protein Asia 1 Type Foot-and Mouth Disease Virus

作　　者：林彤[1] 邵军军[1] 丛国正[1] 独军政[1] 高闪电[1] 常惠芸[1] 谢庆阁[1] 

机构地区：[1]中国农业科学院兰州兽医研究所家畜疫病病原生物学国家重点实验室农业部畜禽病毒学重点实验室,甘肃兰州730046

出　　处：《安徽农业科学》2009年第23期11025-11026,11036,共3页Journal of Anhui Agricultural Sciences

基　　金：国家高技术研究发展计划(863)资助项目(2006AA10A204);中央级公益性科研院所基本科研业务费专项资金项目资助

摘　　要：应用纯化的Asia1型口蹄疫病毒VP1蛋白作为抗原,采用过碘酸钠法标记纯化的单抗,建立了单抗竞争ELISA,检测10份阳性猪口蹄疫血清及200多份阴性血清,试验结果与样品的背景相一致。敏感性试验和重复试验结果表明,该方法具有稳定和敏感等特性,适合Asia1型猪口蹄疫病毒抗体的监测。Using purified VP1 protein Asia 1 type foot-and mouth disease virus as experimental materials, we established monoclonal antibody competitive ELISA via sodium iodate method and detected ten positive porcine foot-and mouth disease serum samples and more than 200 nega- tive serum samples. The results were all consistent with the background of these samples. Sensitivity and repeatability tests show that this method has various advantages like high stability and sensitivity, it is thus used to monitor the antibody of VP1 protein Asia 1 type foot-and mouth disease virus.

关 键 词：Asia1型FMDV VP1单抗 竞争ELISA 

分 类 号：S813.3[农业科学—畜牧学]