人脑胶质瘤中ATM、ATR、Chk1和Chk2基因表达研究  

Expressions of ATM, ATR, Chk1 and Chk2 genes in human glioma

作　　者：周雪梅[1] 乔健[2] 王娆[3] 黄建萍[2] 王慧博[4] 邱裕友[5] 陈永珍[1] 

机构地区：[1]苏州大学医学部解剖学与组织胚胎学系,苏州215000 [2]无锡市第三人民医院儿科,214010 [3]积水潭医院麻醉科,北京100035 [4]哈尔滨医科大学第一临床医学院神经外科,哈尔滨150001 [5]同济大学附属上海第十人民医院放射科,上海200072

出　　处：《中华神经医学杂志》2009年第7期653-657,共5页Chinese Journal of Neuromedicine

摘　　要：目的研究ATM、ATR、Chk1和Chk2在人脑胶质瘤中的表达及其与肿瘤发生的关系。方法采用SYBR^TMGreen实时定量PCR技术检测35例人原发脑胶质瘤组织和10例正常脑组织中的ATM、ATR、Chk1和Chk2的表达水平。结果ART、Chk1和Chk2基因在各级脑胶质瘤中表达较正常脑组织升高，差异均有统计学意义（P〈0．05）。其中，ATR和Chk2基因表达在Ⅱ级、Ⅲ级、Ⅳ级胶质瘤组织之间差异均无统计学意义（P〉0．05），而Chk1在Ⅳ级胶质瘤中的表达较Ⅱ级、Ⅲ级胶质瘤明显升高，差异均有统计学意义（P〈0．05）。ATM基因表达量在正常脑组织和各级脑胶质瘤中差异无统计学意义（P〉0．05）。结论ATR、Chk1和Chk2在人脑胶质瘤中表达上调，说明这些基因可能与人脑胶质瘤的发生有关。其中，Chk1表达与肿瘤恶性程度有关，可作为判别胶质瘤病理级别的辅助指标。Objective To detect the expressions of DNA damage checkpoint genes including A TR, A TM, Chk1 and Chk2 in human primary gliomas and explore their relations with tumor progression. Methods SYBR^TM Green real-time quantitative PCR was performed to detect the expressions of A TR, A TM, Chk1 and Chk2 genes in 35 cases of primary gliomas and 10 of normal brain tissues. Results In glioma tissues of various pathological grades, the expressions of the target genes, with the exception of A TM gene, were significantly increased as compared to those in normal brain tissues （P〈0.05）. Chkl gene expression was significantly higher in grade Ⅳ than in grade Ⅱ and Ⅲ gliomas （P〈0.05）, but no significant differences were found in A TR or Chk2 gene expression between grade Ⅱ, Ⅲ and Ⅳ gliomas （P〉0.05）. Conclusion The up-regulation ofA TR, Chk1 and Chk2 genes in primary glioma suggests their association with the pathogenesis of glioma. Chk1 expression may indicate the malignancy of glioma and help evaluate the pathological grade of glioma.

关 键 词：神经胶质瘤 DNA损伤 细胞周期检控点 ATM ATR CHK1 CHK2 

分 类 号：R686[医药卫生—骨科学]