负载U251抗原树突状细胞增强CIK杀伤作用的实验研究  

Dendritic cells loaded with U251 tumor cell antigen enhance the cytotoxic effect of cytokine- induced killer cells against U251 cell line

作　　者：王新帅[1] 赵勇刚[2] 冯笑山[1] 赵新汉[3] 祁岩超[4] 郭艳珍[1] 

机构地区：[1]河南科技大学第一附属医院肿瘤内科,洛阳471003 [2]河南科技大学第一附属医院神经外科,洛阳471003 [3]西安交通大学第一附属医院肿瘤内科,西安710061 [4]广州医学院临床肿瘤中心,广州510095

出　　处：《中华神经医学杂志》2009年第7期666-669,共4页Chinese Journal of Neuromedicine

摘　　要：目的探讨负载脑胶质瘤细胞株（U251）蛋白抗原的树突状细胞（DC）与细胞因子诱导的杀伤细胞（CIK）混合培养后对U251细胞杀伤活性的影响。方法提取U251的肿瘤抗原，采用脐血单个核细胞（CBMCs）诱导DC和CIK，用负载肿瘤抗原的DC和CIK共同培养诱导为Ag-DC-CIK；通过形态学和免疫分子表型鉴定成熟的DC；采用MTT法检测CBMCs、CIK、Ag-CIK和Ag-DC—CIK对U251的杀伤活性。结果成熟的DC高度表达CD86（82．66％）、CD40（69．40％），中度表达CD83（57．49％）和CD80（51．14％）；Ag—DC-CIK对U251细胞的杀伤活性（58．78±6．56％）高于CBMCs（29．71±6．99％）、CIK（39．89±9．42％）、Ag—CIK（49．92±4．34％），差异均有统计学意义（P〈0．05）。结论负载U251抗原的DC能增强CIK细胞对靶细胞的杀伤活性，为颅内肿瘤的免疫治疗提供了新思路。Objective To study the cytotoxic effect of cytokine-induced killer cells （CIKs） cocultured with U251 tumor cell antigen-loaded mature dendritic cells （DCs） against U251 cell line. Methods The DCs and CIKs were derived from the cord blood mononuclear ceils （CBMCs） of the same donor. The DCs were challenged with U251 tumor cell antigen, and cocultured with the CIKs to induce the cell complex Ag-DC-CIK. The mature DCs were identified by morphological and phenotypic analyses. MTT assay was performed to detect the cytotoxic effects of CBMCs, CIKs, antigen-loaded CIKs （Ag-CIK） or the cell complex Ag-DC-CIK in U251 cells. Results The mature DCs derived from the CBMCs highly expressed the costimulatory molecules CD86 （82.66%） and CD40 （69.40%）, and moderately expressed CD83 （57.49%） and CDS0 （51.14%）. The cytotoxic activity of the cell complex Ag-DC-CIK against U251 cells （58.8%） was significantly higher than those of CBMCs （29.71%）, CIKs （39.89%）, and Ag-CIK （49.92%）. Statistical analysis indicated significant difference in the cytotoxic activity between any two of the groups （P〈0.05）. Conclusion The DCs loaded with the tumor cell antigen can enhance the cytotoxic effect of the CIKs against the target tumor cells, which sheds light on a new approach of immunotherapy for intracranial tumors.

关 键 词：神经胶质瘤 树突状细胞 杀伤细胞 

分 类 号：R686[医药卫生—骨科学]