半乳糖凝集素3及骨桥蛋白在非小细胞肺癌亚临床转移中的意义  

The expression of galectin-3 and steopontin in occult metastasis of non-small cell lung cancer

作　　者：梁勇[1] 李辉[1] 侯生才[1] 胡滨[1] 苗劲柏[1] 李彤[1] 游宾[1] 于立新[1] 王磊[1] 陈其瑞[1] 陈星[2] 

机构地区：[1]首都医科大学附属北京朝阳医院胸外科,100020 [2]首都医科大学基础医学中心,100020

出　　处：《中华外科杂志》2009年第14期1061-1063,共3页Chinese Journal of Surgery

基　　金：北京市教育委员会科技发展计划面上项目（km200710025019）

摘　　要：目的研究半乳糖凝集素-3（Gal-3）和骨桥蛋白（OPN）在非小细胞肺癌（NSCLC）中的表达及其与亚临床转移的关系。方法选取2006年1月至2007年10月接受外科治疗的NSCLC患者46例，男性28例，女性18例，年龄33—77岁。应用RT—PCR及ELISA分别检测患者肺癌组织和血浆中Gal-3和OPN的表达，并结合术后随访，对肿瘤转移组和非转移组进行比较。结果46例NSCLC患者中，12例术后出现肿瘤转移。转移组血浆Gal-3水平为（18．8±7．9）ug/L，高于非转移组的（9．2±5．6）ug/L。转移组血浆OPN为（153．5±63．5）ug/L，高于非转移组的（89．2±24．0）ug/L。转移组癌组织中Gal-3 mRNA含量为0．73±0．23，OPN mRNA含量为0．79±0．24，均高于非转移组（0．07±0．17和0．17±0．25）。结论Gal-3和OPN是NSCLC的一种重要负性预后因素。血浆中Gal-3和OPN的高表达有可能成为判断NSCLC亚临床转移的有效指标之一。Objective To study the relationship between expression of galectin-3 （Gal-3） and osteopontin （OPN） in occult metastasis in non-small cell lung cancer. Methods Forty-six patients of non-small cell lung cancer （NSCLC） from January 2006 to October 2007 were selected. There were 28 males and 18 females, aged from 33 to 77 years old. The levels of lung tissues Gal-3 and OPN were detected by RT-PCR, and the levels of blood plasma＇s were measured by ELISA. Results There were 12 patients who had metastasized. In un-metastasis group the Gal-3 and OPN mRNA expression levels were significantly lower than that in metastasis group： mean value were 0. 07 ±0. 17 and 0. 17±0.25 in un-metastasis group, while 0. 73 ~ 0. 23 and 0. 79 ± 0. 24 in metastasis group. Blood plasm levels of Gal-3 （ 18. 8 ± 7. 9 ）ug/L and OPN （ 153.5± 63.5 ）ug/L in NSCLC which were detected from metastasis group were higher than un-metastasis group of （9. 2 ± 5.6 ） ug/L and （ 89.2 ± 24. 0 ） ug/L. Conclusions High serum levels of Gal-3 and OPN and high expression of Gal-3 and OPN mRNA in NSCLC are closely related to the occurrence and metastasis of NSCLC. They may be indexes of evaluating the occult metastasis in NSCLC.

关 键 词：癌 非小细胞肺 肿瘤转移 半乳糖凝集素3 骨桥蛋白质 

分 类 号：R686[医药卫生—骨科学]