肺出血新生大鼠肺组织组胺水平的变化  

Change of histamine level in the lungs of newborn rats with pulmonary hemorrhage

作　　者：吴春英[1] 游上游[1] 鲁建生[1] 胡红兵[1] 康世秀[1] 周泉[1] 孙金枝[1] 

机构地区：[1]武汉市儿童医院,湖北武汉430016

出　　处：《临床儿科杂志》2009年第7期670-672,共3页Journal of Clinical Pediatrics

基　　金：武汉市卫生局临床重点学科研究项目(No.武卫[2004]241号)

摘　　要：目的观察肺出血新生大鼠肺组织组胺水平的动态变化,以探讨新生儿肺出血的发病机制。方法新生大鼠40只随机分为4组,即实验1组、2组、3组和正常对照组,每组10只。实验1、2、3组大鼠背部皮下注射0.1%肾上腺素0.5ml/kg后放4℃冷室。1组大鼠1h取出,断头处死;2组4h取出,断头处死;3组4h取出后复温,同时注射肾上腺素1次,剂量不变,间隔1h再注射1次,剂量加倍,出冷室2h断头处死。肉眼观察肺组织大体病理情况将结果分为5级:Ⅰ级正常肺,Ⅱ级肺水肿,Ⅲ级点状肺出血,Ⅳ级局灶性肺出血,Ⅴ级弥漫性肺出血。用荧光分光光度法测定肺组织组胺水平。结果肺大体病理改变:1组Ⅱ级肺9例,Ⅴ级肺1例;2组Ⅱ级肺7例,Ⅲ级肺2例,Ⅴ级肺1例;3组Ⅲ级肺2例,Ⅳ级肺1例,Ⅴ级肺7例。组间平均RIDIT分析,差异有高度统计学意义(P<0.01)。实验3组肺组织组胺水平分别与实验1组、2组及对照组比较明显升高(P<0.05)。结论在新生大鼠肺出血的形成过程中,肺组织组胺水平明显升高,组胺能使血管通透性增加,与肺出血的发生可能有密切相关性。Objective To observe the levels of histamine in the lungs of newborn rats with pulmonary hemorrhage for exploration of the pathogenesis of pulmonary hemorrhage in newborn infants. Methods Forty newborn rats were divided randomly into four groups, three experimental groups （group 1, 2, 3） and normal control group （n = 10 respectively）. The rats of group 1, 2, 3 were subcutaneously injected with 0.1% epinephrine at 0.5 mL/kg and put into 4℃ refrigerator. The group 1 rats were taken out of the refrigerator after 1 h and killed. The group 2 and group 3 rats were taken out after 4 h. The group 2 rats were killed. The group 3 rats were warmed and given with the same amount of epinephrine as before and 1 h later the double amount of epinephrine was administered. After 1 h they were killed. Gross anatomical findings of the lungs were divided into five grades, Grade Ⅰ （normal lung）, Grade Ⅱ （pulmonary edema） , Grade m （spotty pulmonary hemorrhage）, Grade Ⅳ （local pulmonary hemorrhage） and Grade Ⅴ （diffuse pulmonary hemorrhage）. The levels of histamine in the lungs were measured by fluorometric method. Results The pathologic changes of the lungs were revealed, 9 cases of Grade Ⅱ and 1 case of Grade Ⅴ in group 1 ; 7 cases of Grade Ⅱ , 2 cases of Grade Ⅲ and Ⅰ case of Grade Ⅴ in group2; 2 cases of Grade Ⅲ, 1 case of Grade Ⅳ and7 cases of Grade Ⅴ in group 3; 10cases of Grade Ⅰ in control group. By the RIDIT analysis a significant difference was found among groups （P 〈 0.005）. The mean level of histamine was significantly higher in group 3 than in group 1, group 2 and control group （P 〈 0.05）. Conclusions During pulmonary hemorrhage period the levels of histamine were increased. Histamine can increase permeability of vessels, which might be closely related to pulmonary hemorrhage.

关 键 词：新生大鼠 组胺 肺出血 

分 类 号：R722.1[医药卫生—儿科]