单人操作建立小鼠原位肝移植模型  

Modified technique for construction of orthotopic liver transplantation model in mice

作　　者：黄德荣[1] 吴忠均[1] 郑树森[2] 朱宇[1] 

机构地区：[1]重庆医科大学附属第一医院肝胆外科,重庆400016 [2]浙江大学医学院附属第一医院外科,浙江杭州310003

出　　处：《中国普通外科杂志》2009年第7期677-680,共4页China Journal of General Surgery

基　　金：国家自然科学基金资助项目(30772055);重庆市卫生局科研项目(06-2-017)

摘　　要：目的探讨一种能稳定、高效地建立小鼠原位肝移植模型且能单人操作的手术改良方法。方法参照"双袖套"法,肝上下腔静脉采用连续缝合,门静脉、肝下下腔静脉采用袖套法,胆管用支架法吻合,完成小鼠原位肝肝移植70例。术后观察24h,1周和1个月受体存活率,肝功能及肝组织病理变化。并设立假手术组对照。结果受体术后24h,1周和1个月存活率分别为95.7%,90.9%和85.1%。术后1周ALT逐渐升高,1个月降至正常水平;术后ALP值逐渐增高;病理显示肝组织结构良好。结论该方法成活率高,稳定性好,重复性强,是建立小鼠原位肝移植模型的理想方法。Objective To study the surgical technique of construction of orthopic liver transplantation model in mice by a single operator. Methods On the basis of two-cuff technique, running suture was used to reestablish the suprahepatic vena cava （ SHVC ） , ＂ two-cuff＂ technology to reestablish the portal vein （ PV ） and infrahepatic vena cava（ IHVC ） , and＂ stent＂ to reestablish the bile duet. Operation was performed in 70 mice, the 24 h, 1 week, 1 M postoperative survival rate were noted, and hepatic function and pathological change were observed. Results The 24 h,1 week and 1M survival rate was 95.7%, 90.9% , 85.1%, respectively. The ALT increased gradually in the first postoperative week, and dropped to normal level in the first month. Pathology showed the structure of liver tissue was fine. Conclusions The method is an ideal mothod to establish the orthotopic liver transplantation model in mice, because it has high survival rate, good stability and is easily replicated.

关 键 词：肝移植/方法 模型 动物 小鼠 

分 类 号：R617[医药卫生—外科学]