姜黄素抑制大鼠肝纤维化的研究  

Study of curcumin inhibition of hepatic fibrosis in rats

作　　者：黄建贤[1] 朱宝和[1] 贺德[1] 黄林[1] 胡钶[1] 侍立志[1] 

机构地区：[1]广东省深圳市宝安人民医院外一科,广东深圳518101

出　　处：《中国普通外科杂志》2009年第7期723-726,共4页China Journal of General Surgery

摘　　要：目的探讨姜黄素抑制肝纤维化的效应及其分子机制。方法培养大鼠肝星状细胞(HSC)并以刀豆蛋白(ConA)激活,再用不同剂量姜黄素处理;建立大鼠四氯化碳(CCl4)肝纤维化模型,以不同剂量姜黄素处理各模型动物,检测其血清透明质酸(HA)、层粘连蛋白(LN)、Ⅲ型前胶原(PCⅢ)和Ⅵ型胶原(Ⅵ.C)水平,检测肝组织纤维化程度和肝组织及HSC基质金属蛋白酶-2(MMP-2)的表达。结果CCl4成功诱导肝纤维化大鼠模型。与模型组比较,姜黄素治疗后能降低血清肝纤维化各项指标(HA,LN,PCⅢ及Ⅵ.C)的表达(P<0.05),肝组织纤维化评分明显降低(P<0.05)。姜黄素处理后,纤维化大鼠肝组织MMP-2呈剂量依赖性减少(P<0.05),活化肝星状细胞MMP-2的表达呈剂量依赖性减少(P<0.05)。结论姜黄素可通过减少MMP-2表达抑制大鼠肝纤维化。Objective To investigate the effect of curcumin on hepatic fibrosis （HF） in rats and its molecular mechanism. Methods Rat hepatic stellate cells were cultured and activated with ConA and processed with different concentration of curcumin. Models of hepatic fibrosis in rats induced by subcutaneous injection of CCl4 were treated with different concentrations of curcumin. The levels of HA, LN, PC Ⅲ and IV. C in serum and histological changes in liver tissues were determined to reveal the extent of liver fibrosis. Matrixmetalloproteinase-2 （ MMP-2 ） expression in liver tissues and HSC was determined by Western blot and RT-PCR. Results Curcumin significantly reduced the levels of HA, LN, PC m and IV. C in serum of HF rats （ P 〈 0.05 ） . the score of hepatic fibrosis in curcumin group was reduced significantly （ P 〈 0.05 ） , Curcumin also inhibited MMP-2 expression in liver tissues （ P 〈 0. 05 ） and activated HSC in a dose-dependent way （ P 〈 0.05 ）. Conclusions Curcumin can inhibit hepatic fibrosis in rats by reducing MMP-2 expression.

关 键 词：肝纤维化 姜黄素 MMP-2 模型 动物 

分 类 号：R657.3[医药卫生—外科学]