CGRP对内皮素-1诱导肝细胞损伤的保护作用  

The protective effect of CGRP on ET-1 induced injury of human hepatocyte

作　　者：聂盛丹[1] 李灼日[2] 曹友德[1] 吕品[2] 石泳中[1] 梁珊[1] 

机构地区：[1]湖南省人民医院临床医学研究所 [2]湖南省人民医院肝胆外科,湖南长沙410005

出　　处：《中国普通外科杂志》2009年第7期731-734,共4页China Journal of General Surgery

基　　金：湖南省科技厅重点资助项目(2008sk2004)

摘　　要：目的探讨内皮素1(ET-1)对人肝细胞的损伤作用及降钙素基因相关肽(CGRP)的保护作用与机制。方法外科切除肝组织分为:正常对照组(D-Hanks液灌注);ET-1(10-7M)灌注损伤组;CGRP(10-6M,10-7M,10-8M)干预组。采用胶原酶灌注法分离肝细胞,检测肝组织中肿瘤坏死因子α(TNF-α)及丙二醛(MDA)含量,台盼蓝检测新鲜分离肝细胞成活率,并体外培养肝细胞。用MTT法观察肝细胞活力,收集细胞培养上清检测谷丙转氨酶(ALT)、白(清)蛋白(Alb),尿素(Urea)和乳酸脱氢酶(LDH)水平。结果与对照组比较,ET-1组肝细胞成活百分率及活力,ALB合成及Urea代谢显著减低(P<0.05);肝组织中TNF-α及MDA含量,LDH及ALT释放增加(P<0.05);而CGRP(10-6M,10-7M,10-8M)能显著减轻ET-1诱导的肝细胞损伤,使肝细胞成活率及活力、Alb分泌及Urea代谢均显著增加(P<0.05),使LDH及ALT,TNF-α及MDA显著减少(P<0.05)。结论CGRP对ET-1诱导的肝细胞损伤具有保护作用,其机制与MDA和TNF-α释放减少有关。Objective To investigate the protective effect of Calcitonin Gene-related Peptide （CGRP） on ET-1 mediated injury of human hepatocyte. Methods Human liver tissues obtained from patients undergoing partial hepatectomies were randomly divided into five groups ： control group, liver perfused with D- Hank＇ s solution group ; liver perfused with ET- 1 group and three liver perfased with ET plus CGRP （ 10^- 6 M, 10^ -7 M,10^-8 M） treated groups. Collagenase digestion method was used to isolate human hepatocytes, then hepatocytes were cultured, and the level of MDA and TNF-α, the viability and proliferation of hepatocyte, and the hepatocyte function （ALT, Alb, Urea and LDH ） were determined. Results As compared with control group, in ET-1 group, the viability and proliferation of hepatocytes ,. the level of Alb and Urea declined significantly （ P 〈 0. 05 ） , and the concentration of ALT, LDH, MDA and TNF-α increased markedly （ P 〈 0.05 ） . In CGRP groups, the viability and proliferation of hepatocytes, the level of Alb and Urea increased significantly （ P 〈 0.05 ） , the concentration of ALT and LDH, the level of TNF-α and MDA markedly decreased with concentration （ all P 〈 0.05 ） . Conclusions CGRP has protective effect on injury of heoatocvtes induced by ET-1 , which may be through decrease the release of MDA and TNF-α.

关 键 词：肝细胞 内皮素-1 降钙素基因相关肽 

分 类 号：R657.3[医药卫生—外科学]