弓形虫可溶性速殖子抗原联合IFNγ滴鼻免疫小鼠诱导的免疫应答  

Immune Response Induced by Intranasal Immunization with STAg and IFNγ Adjuvant in Mice

作　　者：弓鸿飞[1,2] 刘红丽[1] 孟晓丽[1] 刘转转[1] 殷国荣[1] 

机构地区：[1]山西医科大学医学寄生虫学研究所寄生虫学教研室,太原030001 [2]山西医科大学汾阳学院,山西汾阳032200

出　　处：《中国生物制品学杂志》2009年第7期682-685,共4页Chinese Journal of Biologicals

基　　金：国家自然科学基金(30640057);山西省青年科技基金(20051045)

摘　　要：目的观察弓形虫可溶性速殖子抗原(STAg)联合IFNγ滴鼻免疫BALB/c小鼠诱导的免疫应答,为研制弓形虫黏膜疫苗提供实验依据。方法将BALB/c小鼠随机分为实验组和对照组,实验组以STAg(20μg/只)+IFNγ(1 000 U/只)滴鼻,对照组以PBS滴鼻。免疫2次,间隔2周。分别于初次免疫后0、2、4、6、8、10、12周处死小鼠,分离脾淋巴细胞、肠上皮内淋巴细胞(IELs)并计数;分离血清,用ELISA法测定IgA和IgG含量。结果免疫后实验组小鼠IELs和脾淋巴细胞均有增生,IELs和脾淋巴细胞数量均于初次免疫后4周达峰值,IEL数量4、6周显著高于对照组;脾淋巴细胞数量2、4周显著高于对照组。实验组小鼠血清IgG和IgA水平均于初次免疫后4周达峰值,IgG水平2、4周显著高于对照组,至12周时仍高于对照组;IgA水平4、6周显著高于对照组。结论STAg联合IFNγ滴鼻免疫BALB/c小鼠,可有效诱导黏膜及系统免疫应答,且可持续较长时间。Objective To observe the immune response induced by intranasal immunization with soluble tachyzoite antigen （STAg） and IFNγ adjuvant in mice. Methods Eighty-four BALB/c mice were divided into test and control groups randomly, 42 for each. The mice in test group were immunized with 20 μg STAg plus 1000 U IFNγ by intranasal drip for 2 times at an interval of 2 weeks, while those in control group with PBS. Kill 6 mice 0, 2, 4, 6, 8, 10 and 12 weeks after the first immunization respectively, count the splenic lymphoeytcs and intestinal intraepithelial lymphocytes （IELs）, and determine the serum tgA and IgG levels by ELISA. Results Both the splenic lymphocytes and IELs of mice in test group proliferated to peak values 4 weeks after the first immunization. The counts of IELs were significantly higher in test group than in control group 4 and 6 weeks, while those of splenic lymphocytes 2 and 4 weeks after the first immunization. Both serum IgG and IgA levels in test group reached peak values 4 weeks after the first immunization. The IgG level in test group was significantly higher 2 and 4 weeks, while still higher 12 weeks after the first immunization, than that in control group. The IgA level was significantly higher in test group than in control group 4 and 6 weeks after the first immunization. Conclusion Intranasal immunization with STAg and IFNγ adjuvant effectively induced persistent mucosal and systemic immune responses in mice.

关 键 词：弓形虫 可溶性速殖子抗原 干扰素Γ 滴鼻免疫 

分 类 号：R382.5[医药卫生—医学寄生虫学]