Hybrids between olive flounder Paralichthys olivaceus and stone flounder Kareius bicoloratus: karyotype,allozyme and RAPD analyses  

作　　者：尤锋 王伟 徐冬冬 朱香萍 倪静 吴志昊 徐永立 王新成 张培军 

机构地区：[1]Institute of Oceanology,Chinese Academy of Sciences [2]Qingdao Agricultural University

出　　处：《Chinese Journal of Oceanology and Limnology》2009年第2期317-323,共7页中国海洋湖沼学报（英文版）

基　　金：Supported by National Natural Science Foundation of China (No. 30571445);National High-Technology Research and Development Program (863 Program, No. 2006AA10A404);Project from the Ministry of Science and Technology of China (No. 2006DKA30470-017)

摘　　要：The hybrid between olive flounder Paralichthys olivaceus and stone flounder Kareius bicoloratus was produced by artificial insemination of olive flounder eggs with stone flounder sperm. Sinistral and dextral are two types of hybrid progeny after metamorphosis. Karyotypes of both hybrid flounders are the same as those of the two parental species. Of the 22 loci examined from 12 allozymes, 12 confirmed hybridization of the paternal and maternal loci in hybrids and no difference was found in allozyme patterns of sinistral and dextral hybrid fishes. RAPD patterns of these specimens were also studied with 38 primers selected from 104 tested. Among them, the PCR products of 30 primers showed hybridization of the paternal and maternal bands. Genetic variation between hybrids and their parental stocks was analyzed by RAPD using 10 of the above 38 primers. The average heterozygosity and genetic distance were calculated. The results suggested that the filial generation could inherit a little more genetic materials from paternal fish than that from maternal fish.The hybrid between olive flounder Paralichthys olivaceus and stone flounder Kareius bicoloratus was produced by artificial insemination of olive flounder eggs with stone flounder sperm. Sinistral and dextral are two types of hybrid progeny after metamorphosis. Karyotypes of both hybrid flounders are the same as those of the two parental species. Of the 22 loci examined from 12 allozymes, 12 confirmed hybridization of the paternal and maternal loci in hybrids and no difference was found in allozyme patterns of sinistral and dextral hybrid fishes. RAPD patterns of these specimens were also studied with 38 primers selected from 104 tested. Among them, the PCR products of 30 primers showed hybridization of the paternal and maternal bands. Genetic variation between hybrids and their parental stocks was analyzed by RAPD using 10 of the above 38 primers. The average heterozygosity and genetic distance were calculated. The results suggested that the filial generation could inherit a little more genetic materials from paternal fish than that from maternal fish.

关 键 词：artificial hybridization Paralichthys olivaceus （Temminck et Schlegel） Kareius bicoloratus （Basilewsky） KARYOTYPE ALLOZYME RAPD 

分 类 号：S917.4[农业科学—水产科学]