Expression and localization of a novel phosducin-like protein from amphioxus Branchiostoma belcheri  

作　　者：萨仁高娃 赵永刚 

机构地区：[1]Department of Marine Biology,Ocean University of China [2]Key Laboratory of Marine Geology and Environment,Institute of Oceanology,Chinese Academy of Sciences [3]China Animal Health and Epidemiology Center

出　　处：《Chinese Journal of Oceanology and Limnology》2009年第2期324-330,共7页中国海洋湖沼学报（英文版）

基　　金：Supported by the Pilot Projects of Knowledge Innovation Project of Chinese Academy of Sciences (No. KZCX2-YW-211-03 and MGE2008KG06)

摘　　要：A full length amphioxus cDNA, encoding a novel phosducin-like protein （Amphi-PhLP）, was identified for the first time from the gut cDNA library of Branchiostoma belcheri. It is comprised of 1 550 bp and an open reading frame （ORF） of 241 amino acids, with a predicted molecular mass of approximately 28 kDa. In situ hybridization histochemistry revealed a tissue-specific expression pattern of Amphi-PhLP with the high levels in the ovary, and at a lower level in the hind grit and testis, hepatic caecum, gill, endostyle, and epipbaryngeal groove, while it was absent in the muscle, neural tube and notochord. In the Chinese Hamster Ovary （CHO） cells transfected with the expression plasmid pEGFP-NI/Amphi-PhLP, the fusion protein was targeted in the cytoplasm of CHO cells, suggesting that Amphi-PhLP is a cytosolic protein. This work may provide a framework for further understanding of the physiological function of Amphi-PhLP in B. belcheri.A full length amphioxus cDNA, encoding a novel phosducin-like protein (Amphi-PhLP), was identified for the first time from the gut cDNA library of Branchiostoma belcheri. It is comprised of 1 550 bp and an open reading frame (ORF) of 241 amino acids, with a predicted molecular mass of approximately 28 kDa. In situ hybridization histochemistry revealed a tissue-specific expression pattern of Amphi-PhLP with the high levels in the ovary, and at a lower level in the hind gut and testis, hepatic caecum, gill, endostyle, and epipharyngeal groove, while it was absent in the muscle, neural tube and notochord. In the Chinese Hamster Ovary (CHO) cells transfected with the expression plasmid pEGFP-N1/Amphi-PhLP, the fusion protein was targeted in the cytoplasm of CHO cells, suggesting that Amphi-PhLP is a cytosolic protein. This work may provide a framework for further understanding of the physiological function of Amphi-PhLP in B. belcheri.

关 键 词：amphioxus B. belcheri Amphi-PhLP northern blotting in situ hybridization cellularlocalization 

分 类 号：Q78[生物学—分子生物学]