Accumulation and depuration of pectenotoxins in brown crab Cancer pagurus  

作　　者：李兆新 

机构地区：[1]Yellow Sea Fishery Research Institute [2]Food technology college,Ocean University of China [3]Norwegian School of Veterinary Science

出　　处：《Chinese Journal of Oceanology and Limnology》2009年第2期389-394,共6页中国海洋湖沼学报（英文版）

基　　金：Supported by Norwegian Education Funding "Quata"(2005)

摘　　要：Pectenotoxins (PTXs) are a group of marine algal toxins. In this study, the accumulation and depuration of pectenotoxins in brown crab Cancer pagurus were investigated. Crabs were fed with toxic blue mussels Mytilus edulis for 21 days and then depurated for 42 days. Toxins were extracted with methanol from the digestive glands of contaminated crabs, uncontaminated crabs (control group) and from blue mussels for comparison. Extracts were analyzed by liquid chromatograph coupled with tandem mass spectrometry (LC-MS-MS). The concentrations of PTX-2, PTX-2 SA, 7-epi-PTX-2 SA, and PTX-12 were analyzed in two batches of toxic blue mussels and the crabs. A one-compartment model was applied to describe the depuration of PTXs. The half-life of PTXs was estimated to be 6–7.5 days. After depuration for 42 days, the amount of PTXs measured in the crab digestive glands was less than 1 μg/kg.Pectenotoxins （PTXs） are a group of marine algal toxins. In this study, the accumulation and depuration of pectenotoxins in brown crab Cancer pagurus were investigated. Crabs were fed with toxic blue mussels Mytilus edulis for 21 days and then depurated for 42 days. Toxins were extracted with methanol from the digestive glands of contaminated crabs, uncontaminated crabs （control group） and from blue mussels for comparison. Extracts were analyzed by liquid chromatograph coupled with tandem mass spectrometry （LC-MS-MS）. The concentrations of PTX-2, PTX-2 SA, 7-epi-PTX-2 SA, and PTX-12 were analyzed in two batches of toxic blue mussels and the crabs. A one-compartment model was applied to describe the depuration of PTXs. The half-life of PTXs was estimated to be 6-7.5 days. After depuration for 42 days, the amount of PTXs measured in the crab digestive glands was less than 1 μg/kg.

关 键 词：pectenotoxins brown crab blue mussel ACCUMULATION DEPURATION Cancer pagurus Mytilus edulis 

分 类 号：S917.4[农业科学—水产科学]