生物可降解材料PLGA90/10的体外降解特性及生物相容性  

In-vitro Degradation of Poly(DL-lactic-co-glycolic acid) 90L/10G

作　　者：杨宁[1] 李为民[1] 李悦[1] 张伟 刘巍[1] 耿建强[1] 邵群[1] 

机构地区：[1]哈尔滨医科大学第一临床医学院心内科,哈尔滨150001 [2]哈尔滨市第五医院骨科,哈尔滨150001

出　　处：《生物医学工程研究》2009年第2期100-103,共4页Journal Of Biomedical Engineering Research

基　　金：黑龙江省科技攻关项目(No.GB06C50101)

摘　　要：研究生物可降解材料聚乳酸乙醇酸共聚物(PLGA 90/10)的体外降解特性,通过测量材料在体外降解过程中的重量损失并在扫描电镜下观察形态学变化,评估体外降解特性,该实验是在37℃pH为7.4的磷酸盐缓冲液中进行的。为研究材料的细胞毒性,将小牛血管平滑肌细胞种植在PLGA膜片上,在显微镜下观察细胞生长情况,并通过MTT法测定细胞活性。研究发现结晶型的PLGA90/10显示出表面降解的特性,高分子量的PLGA90/10在开始的4个月内降解缓慢,120天时重量仅损失10%,120天后迅速降解。MTT法检测细胞增殖指数,与对照组差异无显著意义。这为局部药物治疗工具提供了基础研究。We assessed the in vitro degradation of poly（dl- lactic - co - glycolic acid） （PLGA）90L/10G, a biodegradable material. In vitro degradation characteristics were evaluated by measuring decrease in the mass loss of PLGA fdms and conducted at 37℃ in pH 7.4 phosphate buffered saline. The morphological changes of films surface during degradation were observed on a scanning electron microscopy. The viability of cells was determined by MTT method. PLGA90/10 was hydrophobic polymer and it showed surface erosion characteristics. High - molecular- weight PLGA degraded slowly in the 4 months of beginning; there was 10% mass loss in the 120th day and rapid mass loss after the 120th day. There was no difference on the cell viability between control group and PLGA group. Our study provides a basis for the development of a useful vehicle for locally administered drugs.

关 键 词：聚乳酸乙醇酸共聚物(PLGA) 体外降解 降解速率 细胞毒性 

分 类 号：R318[医药卫生—生物医学工程]