茂名市首例甲型H1N1流感病例的实时荧光RT-PCR检测  

Testing for the First Patient of Influenza A (H1N1) Virus by Real-time RT-PCR in Maoming City

作　　者：廖国东[1] 陈家图[1] 罗光毅[1] 何展[1] 杨适乡[1] 胡乐[1] 

机构地区：[1]茂名市疾病预防控制中心,茂名525000

出　　处：《中国国境卫生检疫杂志》2009年第3期142-144,共3页Chinese Journal of Frontier Health and Quarantine

摘　　要：〔目的〕对茂名市首例甲型H1N1流感病例进行病毒核酸检测,为甲型H1N1流感疫情的防控提供参考。〔方法〕采用实时荧光逆转录PCR法对采集的病人咽拭子标本进行甲型H1N1流感病毒核酸检测。〔结果〕共采集病人2份咽拭子标本,其中1份咽拭子标本的实时荧光RT-PCR检测结果显示H1及N1的RT-PCR反应体系扩增曲线有明显对数增长且Ct值分别为25.21(H1)和25.07(N1),为甲型H1N1流感病毒核酸阳性;另1份咽拭子标本则呈阴性。后采集第3份咽拭子标本连同第1分标本送广东省疾控中心复检,结果2份标本的甲型H1N1流感病毒核酸检测均为阳性。〔结论〕这名病人为福建省第2例甲型H1N1流感病毒感染者的密切接触者,由于及时采用灵敏度高,特异性强,检测时间短的实时荧光逆转录PCR方法检测,快速确诊了病例,为疫情的控制争取了时间,防止了2代病例的出现。Objective To test the Virus nucleic acid for the first patient of Influenza A （H1N1） in Maoming City, and to provide the references for prevention and control of Influenza A （H1N1）. Method The throat swab of patient was collected and tested by Real-time PCR. Result In 2 samples of throat swabs the 1st throat swab had the obvious logarithm increasing for H1 and N1 in reaction system of Real-time RT-PCR, the Ct numeric of H1 and N1 was 25.21 and 25.07, it was positive of Influenza A （HIN1）; but the 2nd throat swab was negative. The 3rd throat swab was sent to Center for Disease Control and Prevention of Guangdong province with 1st throat swab, both the result of re-test of two throat swab showed positive of Influenza A （H1N1）. Conclusion This patient was the close contact the 2nd infecter with Influenza A （H1N1） in Fujian province. This case was identified rapidly by Real-time RT-PCR which has high sensitivity and specificity. Meanwhile, the aggressive and preemptive measures have been taken in a timely manner, and the secondary cases have been successfully prevented.

关 键 词：甲型流感 H1N1亚型 实时荧光RT—PCR 核酸检测 

分 类 号：R511.7[医药卫生—内科学]