参附注射液对大鼠肠缺血再灌注肺损伤的保护作用  

Protective Effects of Shen-Fu Injection on the Lung Injury Induced by Intestinal Ischemia Reperfusion in Rats

作　　者：王进[1] 乔礼芬[2] 李永胜[1] 杨光田[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院急诊科,武汉430030 [2]华中科技大学同济医学院附属同济医院呼吸科,武汉430030

出　　处：《中国药学杂志》2009年第13期993-996,共4页Chinese Pharmaceutical Journal

基　　金：湖北省科技攻关计划项目(2003AA301C51)

摘　　要：目的探讨参附注射液(SF)对大鼠肠缺血-再灌注(II/R)引起的远隔器官肺脏损伤的保护作用。方法通过夹闭肠系膜上动脉60min,再灌注120min,建立II/R损伤模型。实验大鼠随机分为4组:假手术组;II/R组;低剂量SF预处理组(SF-5组,5mL·kg-1);高剂量SF预处理组(SF-10组,10mL·kg-1)。观察各组大鼠肺组织丙二醛(MDA)、髓过氧化物酶(MPO)、肿瘤坏死因子-α(TNF-α)水平,肺脏组织H-E染色,ICAM-1和NF-κB表达。结果II/R组肺组织中MDA、MPO、TNF-α水平明显高于假手术组(P<0.05),ICAM-1与NF-κB表达明显高于假手术组(P<0.05),镜检发现肺组织有明显组织形态学损伤。与II/R组比较,SF能够降低肺组织中MDA、MPO、TNF-α水平(P<0.05);减少肺组织NF-κB和ICAM-1的表达(P<0.05);减轻肺脏组织形态学损伤。结论SF通过抑制NF-κB的激活,减少肺组织ICAM-1的表达和中性粒细胞的聚集,从而减轻II/R引起的肺脏损伤。OBJECTIVE To investigate the protective effects of Shcn-fu injection （SF） on lung injury induced by intestinal ischcmia rcpcrfusion （I/R） in rats. METHODS The intestinal ischcmia rcpcrfusion （I/R） model was established by superior mcscntcric artery （SMA） occlusion. Wistar rats wcrc randomly divided into four groups as follows： （i） control, sham operated group; （ii） I/R group （II/R group）; （iii） group prctrcatcd with 5 mL·kg^-1 Shen-fu injection （SF-5 group）; and （iv） group prctrcatcd with 10 mL·kg^-1 Shen-fu injection （SF-10 group）. Lung histology was observed. Myclopcroxidasc （MPO） activity, Tumor necrosis factor α （TNF-α） and malondialdchydc （MDA） level in lung tissues and the immunohistochemical expression of lung ICAM-1 wcrc assayed. In addition, the Western blot of lung NF-κB was performed. RESULTS Compared with the sham-operated control group, lung injury was induced by intestinal I/R, characterized as histological damage including ocdcma, hacmorrhage and infiltration by inflammatory cells. Compared with the control group, MPO activity, TNF-α and MDA content in the lung tissues were increased significantly in the II/R group. Strong positive expression of lung ICAM-1 was observed. Compared with IFR group, administration of 5 and 10 mL·kg^-1 Fu rnarkcdly ameliorated lung injury. Compared with the II/R group, MPO activity, TNF-α and MDA contents in lung tissues wcre decreased significantly following Shcn-Fu prc-treatment, and the expression of lung NF-κB and ICAM-1 was markedly amelinratcd. CONCLUSION The present study reveals that Shen-Fu relieves lung injury induced by intestinal I/R. One possible mechanism responsible for this effect is the inhibition of ICAM-1 expression and neutrophil infiltration by inhibition of NF-κB activity.

关 键 词：参附注射液 肠缺血再灌注 丙二醛 髓过氧化物酶 肿瘤坏死因子-α 核因子-ΚB 细胞间黏附分子-1 

分 类 号：R965[医药卫生—药理学]