转vgb基因银腺杂种杨的耐涝性  

Waterlogging Tolerance of vgb-Transgenic Poplar

作　　者：李义良[1,2] 苏晓华[1] 张冰玉[1] 黄秦军[1] 张香华[1] 

机构地区：[1]中国林业科学研究院林业研究所国家林业局林木培育重点实验室,北京100091 [2]广东省林业科学研究院,广州510520

出　　处：《林业科学》2009年第7期26-31,共6页Scientia Silvae Sinicae

基　　金：国家"948"引进国际先进林业科学技术创新项目"林木基因工程技术引进与创新"(2006-4-C01);国家"863"高新技术课题"高产优质多抗林木花草分子与细胞高效育种技术及品种创制"子课题"高产优质多抗杨树分子与细胞高效育种技术及品种创制"(2006AA100109)

摘　　要：对导入透明颤菌血红蛋白(vitreoscilla hemoglobin,VHb)基因vgb银腺杂种杨进行Southern印记杂交和RT-PCR检测,证实vgb基因已整合到杨树基因组中,并在转录水平上得到表达。在温室对4个转基因株系及对照盆栽扦插苗进行模拟持续淹水胁迫试验,研究淹水条件下植株的生长及生理性状。结果表明,随着淹水时间的延长苗木逐渐死亡,对照植株在淹水17天后死亡率达66.67%,而4个转基因株系死亡率均低于50.00%,其中T81最低,只有25.00%;同时4个转基因株系在淹水胁迫时能够保持较高的叶绿素含量,并且膜质过氧化物MDA含量均低于对照植株;涝渍胁迫下转基因株系生长受抑制程度低于对照植株。说明在淹水胁迫下vgb基因的表达增强了银腺杂种杨叶绿素含量的积累,进而促进了转基因植株的生长,提高了转基因株系对淹水的忍受能力。Southern blot and RT-PCR were used to detect vgb-transgenic Populus alba × P. glandulosa. The results showed that the vgb gene had been integrated into the genome of the poplar and had expressed at transcription level. A flooding resistant test was performed with transgenic poplars and the non-transgenic control in greenhouse. With increasing flooding treatment time, the death rate was increased, however the death rate in transgenic plants was lower than that in the control plants. In seventeen days after waterlogging treatment, the control plant death rate was 66.67 %, while that in transgenic plants was under 50.00 %. Among them, the death rate in transgenic line of I＂81 was the lowest, with 25.00%. Chlorophyll content was higher and MDA content was lower in leaves of the transgenic plants compared with that in the control plants under the flooding treatment. The growth inhibition degree of transgenic plants was lower than control plants under waterlogging stress. The results suggested that under flooding stress, the expression of vgb gene raised chlorophyll accumulation of poplar plants, promoted the growth of transgenic plants, and increased tolerance of the transgenic poplar to waterlogging.

关 键 词：透明颤菌血红蛋白基因(vgb) 耐涝性 银腺杂种杨 

分 类 号：S718.43[农业科学—林学] S718.46