二氧代哌嗪苯磺酰环己脲降糖作用的研究  

Glucose-lowering effect of dioxopiperazinecarbonylphenylsulfonylcyclohexyl urea

作　　者：陆晓华[1] 李伟[2] 张根葆[2] 桂常青[1] 王国光[2] 

机构地区：[1]皖南医学院机能实验中心,安徽芜湖241002 [2]皖南医学院.病理生理学教研室,安徽芜湖241002

出　　处：《皖南医学院学报》2009年第3期167-170,共4页Journal of Wannan Medical College

摘　　要：目的:研究观察二氧代哌嗪苯磺酰环己脲对链脲佐菌素(STZ)诱导的2型糖尿病小鼠血糖血脂的影响以及刺激SMMC7721细胞对3H-2-脱氧葡萄糖吸收影响。方法:以高脂高热量饲料喂养及腹腔注射链脲佐菌素复制小鼠2型糖尿病模型,采用酶比色法测定糖尿病小鼠的空腹血糖、甘油三酯(triglyceride,TG)、胆固醇(cholesterol,CHO)等相关生化指标;利用液闪计数法检测细胞对3H-2-脱氧葡萄糖的吸收。结果:二氧代哌嗪苯磺酰环己脲可明显降低血糖水平,改善甘油三酯、胆固醇(P<0.01);与对照组比较,二氧代哌嗪苯磺酰环己脲可显著增加SMMC7721细胞对葡萄糖的吸收(P<0.01),3H-2-脱氧葡萄糖的吸收值增加近2倍,并且显著增强胰岛素刺激的葡萄糖吸收作用。结论:二氧代哌嗪苯磺酰环己脲有改善2型糖尿病小鼠血糖血脂的作用;并且具有刺激葡萄糖吸收以及增加胰岛素刺激的葡萄糖吸收作用。Objective ： To explore the effects of 1 -（4 - [ 2 -（ 1 - ethyl- 2,3 - dioxopiperazine4-carboxamido ）ethyl ]-phenylsulfonyl）-3 -eyclohexyl urea on the fasting blood glucose and the blood lipemia in the streptozotocin （STZ）-induced diabetic mice and the glucose uptake in SMMC7721 cells. Methods：The animal models of Type 2 diabetes were prepared by nourishment of high calorie and high fat and by injection of STZ. The enzyme colorimetry was used to detect the level of fasting blood glucose,triglyceride （ TG）, cholesterol （CHO） and scintillation was employed to examine the glucose uptake in SMM7721 cells. Results：The drug effectively decreased the blood glucose and improved the metabolism of blood lipemia in the STZ -induced mice. Compared with the control cells, the drug significantly improved the glucose uptake in SMMC7721 cells （P 〈 0.01 ） and the uptake of 2-deoxy-[ 3 H ] glucose in SMMC7721 cells increased by twice, with evident insulin-stimulated glucose uptake （P 〈 0.01 ）. Conclusion：The new drug could modify the blood glucose and lipemia in STZ- induced mice and improve the glucose uptake in SMMC7721 cells,and it may be a potential anti-hyperglycemic agent.

关 键 词：二氧代哌嗪苯磺酰环己脲 SMMC7721细胞 2型糖尿病 

分 类 号：R965[医药卫生—药理学]