定期无偿献血者NK细胞免疫功能的研究  

The effect on NK cells immunity in regular whole blood and platelet donors

作　　者：李桢[1] 熊文[1] 程良红[1] 唐斯[1] 王飞[1] 张艳艳[1] 

机构地区：[1]深圳市血液中心,广东深圳518035

出　　处：《国际输血及血液学杂志》2009年第4期289-291,共3页International Journal of Blood Transfusion and Hematology

基　　金：深圳市科技计划项目（200603201）

摘　　要：目的分析定期无偿全血捐献者、血小板捐献者的NK细胞数量以及细胞毒活性，评价定期捐血对机体NK细胞免疫功能的影响。方法采用流式细胞术对三组人群（定期无偿全血、血小板和首次捐血者，后者为对照组）外周血中NK细胞群的数量进行分析。采用效／靶细胞混合培养的方法，计算出NK细胞对靶细胞的杀伤率。结果全血捐献者NK细胞数量及其对靶细胞的杀伤率与对照组的差异无统计学意义（P〉0.05）；血小板捐献者NK细胞数量较对照组明显增高（P〈0.05），而NK细胞杀伤活性明显降低（P〈0.05）。结论本研究结果说明，全血捐献者献血间隔期较长（〉3个月），机体有足够的时间来恢复NK细胞数量及其细胞毒性，而血小板捐献者献血间隔期短，机体通过NK细胞数量的增加来调节由于其功能降低对机体造成的影响。Objective To analysis NK cell count and cytotoxic activity in regular whole blood and platelet donors and to evaluate their immunity. Methods The NK cell count was analyzed by flow cytometer in peripheral blood in three crowds, viz free regular whole blood, platelet, and the first time whole blood donors. The last crow was the control group. The NK cell cytotoxic activity was analyzed by mixed culture of effecter cell/ target cell. Results The NK cell numbers and its anti-ratio to target cells in free regular whole blood donors had no significant differences with the control group（P〉0.05）. The platelet donors＇ NK cell numbers was significantly increased compared to control group（P〈0.05）, but their NK cell antiratio was significant lower than control group（P〈0.05）. Conclusion Free regular whole blood donation have a long interval （〉 3 months）, and have enough time to recovery their NK cell numbers and cytotoxicity. In contrast, platelet donation interval is shorter than other groups, so they must increase their NK cell numbers to regulate the effect caused by low function.

关 键 词：定期献血 NK细胞 免疫功能 细胞毒性 流式细胞术 

分 类 号：R446.6[医药卫生—诊断学]