乙酰丹参素单硝酸异山梨醇酯的合成及对心肌缺血/再灌注大鼠心脏的保护作用  

Protective effect of Acetyl Danshen Acid Isosorbide Mononitrate on myocardial ischemia/reperfusion injury in rats

作　　者：向卓[1] 景临林[1] 刘文冲[2] 王平安[1] 高峰[2] 孙晓莉[1] 

机构地区：[1]第四军医大学药学系化学教研室,陕西西安710033 [2]第四军医大学基础部生理学教研室,陕西西安710033

出　　处：《第四军医大学学报》2009年第14期1257-1261,共5页Journal of the Fourth Military Medical University

基　　金："重大新药创制"科技重大专项(2009ZX09103)

摘　　要：目的:考察乙酰丹参素单硝酸异山梨醇酯(AcDI-302)对心肌缺血/再灌注(MI/R)大鼠的心肌保护作用,并与单硝酸异山梨醇酯(ISMN)进行比较.方法:常规制备大鼠MI/R(30 min/3 h)模型.随机分为假手术组,MI/R组,(MI/R+ISMN)组,(MI/R+AcDI-302)组.观察各组大鼠缺血30 min再灌注3 h心功能恢复情况和3 h末血清中肌酸激酶(CK)、乳酸脱氢酶(LDH)活性和丙二醛(MDA)的水平及NO的含量.结果:MI/R+AcDI-302组大鼠较ISMN组心肌梗死范围明显减小,血清中CK,LDH活性和MDA浓度也显著降低,左室收缩压(LVSP)、左室等容收缩/舒张期压力上升或下降最大速率(±dp/dtmax)显著提高.结论:AcDI-302对心肌缺血再灌注损伤大鼠的心肌有保护作用,作用强于ISMN.AIM： To study the protective effect of a new compound, Aeetyl Danshen Acid Isosorbide Mononitrate （AcDI-302）, on myocardial ischemia/reperfusion（MI/R） injury and to compare its effect with that of Isosorbide Mononitrate （ISMN）. IVIETHODS： Male Sprague-Dawley rats were subjected to 30min of myocardial isehemia and 3 h of reperfusion. The rats were randomly assigned to SHAM group, treatment group of MI/R ＋ solvent and MI/R ＋ ISMN and treatment group of MI/R ＋ AeDI-302. MI/R models were prepared by temporary occlusion of the left anterior descending coronary artery. Heart rate （HR）, left ventricle maximum systolic pressures （LVSP） and the maximal first derivative of developed pressure（±dp/dtmax） were monitored throughout the experiments. Myocardial infarction （MI） size and serum ereatine kinase （CK） , lactate dehydrogenase （LDH） , nitric oxide（NO） and malondialdehyde（MDA） of the rats were deter- mined at the end of reperfusion. RESULTS： Compared with ISMN, the new compound of AcDI-302 improved the cardiac function by increasing LVSP and ±dp/dtmax, and decreased the size of myocardial infarction, the activities of CK and LDH and the level of MDA. CONCLUSION： Compared with the protective effect of ISMN, the new compound of AcDI-302 has stronger protective effects on MI/R injury in rats.

关 键 词：乙酰丹参素单硝酸异山梨醇酯 丹参素 单硝酸异山梨酯 一氧化氮供体 心肌缺血 再灌注损伤 

分 类 号：R962[医药卫生—药理学]