超声直方图定量评价大鼠肝脏脂肪变性的实验研究  

Quantitative evaluation of rat's fatty liver using ultrasonography histogram

作　　者：张文缓 刘阁玲[2] 张国顺[3] 马利转 

机构地区：[1]武警河北总队医院唐山分院 [2]唐山市工人医院 [3]华北煤炭医学院附属医院,唐山063000

出　　处：《武警医学》2009年第7期590-593,共4页Medical Journal of the Chinese People's Armed Police Force

摘　　要：目的探讨具有病理基础的脂肪肝无创性超声定量诊断标准。方法通过高脂饮食建立大鼠脂肪肝模型,进行超声直方图(Hist-B)测定。选用雄性wister大鼠40只,分成对照组(普通饲料喂养)与实验组(高脂饲料喂养),建立不同程度的脂肪肝模型,分别于4周、6周、8周、10周、12周同步进行肝脏超声及病理检测。应用日本TOSHIBA 350A彩色超声诊断仪Hist-B功能进行灰阶值测定,病理检测采用HE染色。以病理诊断为标准得出脂肪肝的超声量化指标。结果以病理诊断为标准M值分别为正常肝脏(7.99±0.65)、轻度脂肪肝(15.20±0.74)、中度脂肪肝(24.38±2.17)、重度脂肪肝(35.44±2.58);超声直方图测定法优于肉眼观察测定法:前者敏感度88%,特异度93%,准确率90%;后者敏感度76%,特异度87%,准确率80%。结论高脂饮食可以快速建立大鼠脂肪肝模型,超声直方图能定量评价脂肪肝,且能判断肝脏脂肪浸润的程度,为脂肪肝无创性量化诊断提供了可靠依据。Objective To study the non -invasive, quantitative ultrasonic diagnostic criteria of fatty liver by establishing the rat model fed with high fat diet, using ultrasonic histogram（ Hist - B）. Methods The fatty liver rat model was developed. Forty healthy male Wistar rats were randomly divided into two groups： control group （n = 15 ）was fed with normal diet, and model group （n = 25 ） was fed with highfat diet. The rat＇s liver was monitord by ultrasonography and hi stopathology at weeks 4,6,8,10, and 12, to the detect average value of gray - scale by Hist - B of Toshiba 350A computed sonographer. Histopathological staining method was H＆E. Quantitative ultrasonic diagnostic criteria were calculated. Results According to the pathological findings in the liver, the average value of gray - scale was 7.99 ± 0. 65 in normal group, 15.20 ± 0. 74 in mild degree fatty degeneration group ,24.38 ±2.17 in medium degree fatty degeneration group, and 35.44 ± 2.58 in severe degree fatty degeneration group. Uhrasonography histogram assay was superior to macroscopic observation. The former had a sensitivity of 88%, a specificity of 93%, and an accuracy of 90%, while the latter had 76%, 87%, and 80%, respectively. Conclusions Rat fatty liver models can be developed in a short time by high fat diet. Ultrasonic histogram can reflect the fatty liver disease and judge the fat degree in liver. So it may serve as a reliable non - invasive tool for the quantitative diagnosis of fatty liver disease and judge its severity.

关 键 词：脂肪肝 超声检查 直方图 灰阶值 组织病理学 大鼠 

分 类 号：R445.1[医药卫生—影像医学与核医学]