Ang-1对高糖中培养的RF/6A的影响  

Effects of angiopoietin-1 on RF/6A in high glucose environment

作　　者：左中夫[1] 王轶晶[2] 冯闯[3] 刘学政[4] 

机构地区：[1]辽宁医学院,病理生理教研室,121001 [2]辽宁医学院新药研究所,121001 [3]辽宁医学院研究生学院,121001 [4]辽宁医学院人体解剖与组织胚胎学教研室,121001

出　　处：《中国实用医药》2009年第8期1-2,共2页China Practical Medicine

基　　金：辽宁省优秀青年科研人才培养资金资助项目(项目编号:3050007);辽宁省科技计划资助项目(项目编号:2003225007)

摘　　要：目的探讨血管生成素-1(angiopoietin-1,Ang-1)对高浓度葡萄糖中培养的猴脉络膜-视网膜内皮细胞(RF/6A)的保护作用。方法RF/6A分3组培养:对照组(DMEM培养基含25mmol/L葡萄糖)、高糖组(DMEM培养基含40mmol/L葡萄糖)、Ang-高糖组(DMEM培养基含40mmol/L葡萄糖+0.25mg/LAng-1)。利用台盼兰染色法及MTT比色法检测体外培养的各种RF/6A存活率及细胞活力,利用western-blotting法检测各组suivivin表达。结果1d时各组细胞存活率及活力无差异。3、5、7d时高糖组和Ang-高糖组细胞存活率及细胞活力均较正常组下降(P<0.05),高糖组细胞存活率及细胞活力低于Ang-高糖组(P<0.05)。5d时,Ang-高糖组survivin表达明显较其他两组增多(P<0.05)。结论高糖能降低RF/6A的存活率和活力,而Ang-1能明显增强高糖环境中RF/6A的存活率和活力,其机制可能与Ang-1上调凋亡抑制基因survivin的表达有关。Objective To explore the damage of high glucose on rhesus macaque choroids-retinal endothelial cell（RF/6A） and also evaluate the protective effect and mechanisms of Angiopoietin-1 （Ang-1） on RF! 6A in this environment. Methods RF/6A were divided into 3 groups to be cultured： （ 1 ） control group （DMEM containing 25 mmol/L glucose） ; （2） high glucose group （DMEM containing 40 mmol/L glucose） ; （ 3 ） Ang-high glucose group （ DMEM containing both 40 mmol/L glucose and 0. 25 mg/L Ang-1 ）. Each group were evaluated by measurement of MTT, trypan blue dye exclusion method at the 1 st, 3rd ,5th and 7th day respectively, and evaluated by western-blotting at the 5th day to determine the expression of survivin. Results At the 3 rd, 5 th and 7 th day, lower cell proliferation and livability in high glucose group and Ang-high glucose group were showed, compared with control group （ P 〈 0. 05 ） , the same tendency was observed in high glucose group compared with Ang-high glucose group （ P 〈 0. 05 ）. The western-blotting showed the expression of survivn in Ang-high glucose group was significantly higher than other two groups （ P 〈 0. 05 ）. Conclusion High glucose can decrease the proliferation and livability of RF/6A while Ang-1 limits these damages in high glucose environment may by way of up regulating the expression of survivin.

关 键 词：血管生成素-1 高糖 SURVIVIN表达 

分 类 号：R587.2[医药卫生—内分泌] R774.1[医药卫生—内科学]