T-RFLP技术及其在微生物群落结构研究中的应用  

T-RFLP Analysis Technique and its Application on Analyzing Microbial Community Structure

作　　者：罗佳捷[1,2] 李丽立[1] 张彬[2] 

机构地区：[1]中国科学院亚热带农业生态研究所,长沙410125 [2]湖南农业大学经济动物研究所,长沙410128

出　　处：《中国畜牧兽医》2009年第7期75-78,共4页China Animal Husbandry & Veterinary Medicine

基　　金：国家自然科学基金资助项目(30571351);湖南省自然科学基金(08JJ3080);长沙市科技攻关项目(K-0802117-21)

摘　　要：作者介绍了末端限制性片段长度多态性(T-RFLP)分析技术的基本原理和操作方法,并就该方法在生态环境中、动物和人体内微生物群落结构的研究应用情况做了综述。由于T-RFLP分析技术不依赖于细菌培养,并且整合了自动测序仪的高分辨率和高通量特征,因此,对于分析复杂的群落结构比其它的指纹识别技术具有更明显的优势,最后分析了此种技术的不足之处并对它的应用前景做出了展望。The advance of terminal restriction fragment length polymorphism（T-RFLP） in analyzing microbial community structure in environment,animal and human as well as the fundamental principle and operational approach of T-RFLP were detailed. T-RFLP has many advantages over the other genetic fingerprinting profiles on analyzing microbial community structure owning to its higher resolving power and higher through output by integrated with automatic sequencers and its culture-independent. In addition, the disadvantages and the foreground of this technique were summarized.

关 键 词：末端限制性片段长度多态性 微生物群落结构 指纹识别 自动测序 

分 类 号：Q78[生物学—分子生物学]