不同分割液对奶牛不同阶段胚胎分割效果的影响  

Effects of Different Partition Liquid on Dairy Cow Embryos Segmentation at Different Stages

作　　者：韩永胜[1] 佟桂芝[1] 付龙[1] 

机构地区：[1]黑龙江省畜牧研究所,齐齐哈尔161005

出　　处：《中国畜牧兽医》2009年第7期122-124,共3页China Animal Husbandry & Veterinary Medicine

摘　　要：本试验探讨了3种不同分割液对奶牛桑葚胚和囊胚分割效果的影响。借助显微操作仪,将发育至第6～8天的体内常规生产的桑葚胚和囊胚进行分割,体外培养半胚,观察其发育情况,选择形态恢复好的半胚与一个囊胚滋养层细胞囊泡(trophoblastic vesicles,TRV)共移植。结果显示,在PBS+0.2mol/L蔗糖与PBS+5%PVP中分割桑葚胚,其分割成功率显著高于PBS(P<0.05),分别为89.13%、86.73%和69.67%,而半胚的囊胚发育率及移植妊娠率三者均无显著差异(P>0.05);在PBS+0.2mol/L蔗糖与PBS+5%PVP中分割囊胚,其分割成功率显著高于PBS(P<0.05),分别为94.52%、92.52%和70.52%,而半胚培养的囊胚发育率及移植妊娠率三者均无显著差异(P>0.05);说明在PBS中分别添加0.2mol/L的蔗糖和5%的PVP有利于提高奶牛桑葚胚和囊胚的分割成功率。In this experiment, morula and blastocyst were bisected in three different partition liquid with mieromanipulator. Demi-embryos were cultured and then the development rate was observed. Bisected embryos were co-transferred with trophoblastic vesicles （TRV）. The results showed that morula were bisected in PBS ＋0.2 mol/L sucrose and PBS＋5% PVP,splitting success rate significantly higher than in PBS （89. 13%, 86. 73% VS 69. 67%） （P〈0. 05）, and the blastocysts rate and pregnancy rate were no significant difference （P〉0.05）; in PBS ＋0.2 mol/L sucrose and PBS＋5% PVP in the bisection of blastocysts,splitting success rate significantly higher than in PBS （94.52%,92.52% VS 70.52%） （P〈0.05）, the blastocysts rate and pregnancy rate were no significant difference （P〉0.05） ; the PBS were added in 0.2 mol/L sucrose and 5% PVP will help to improve splitting success rate of the dairy cow morula and blastocyst.

关 键 词：奶牛 胚胎分割 囊胚发育率 TRV 共移植 妊娠率 

分 类 号：S814.8[农业科学—畜牧学]