一种新型生防细菌菌株13-1鉴定及其生物学特性  

Identification and Biological Characteristics on a Novel Strain 13-1 from Rhizosphere of Amorphophallus konjac

作　　者：姬广海[1] 魏兰芳[2] 吴亚鹏[1] 

机构地区：[1]云南农业大学农业生物多样性应用技术国家工程中心,云南昆明650201 [2]云南农业大学农科基础实验教学中心,云南昆明650201

出　　处：《微生物学通报》2009年第7期974-980,共7页Microbiology China

基　　金：国家自然科学基金项目(No.30760139);农业部公益性(农业)行业科研专项(No.nyhyzx07-056)

摘　　要：13-1是从魔芋根际土壤中筛选得到的一株革兰氏阴性生防细菌,抑菌试验结果表明它对7种植物病原细菌和8种病原真菌具有拮抗作用。形态和常规生理生化特征鉴定,发现该菌株与溶杆菌属Lysobacter特征很相近。Biolog鉴定和16S rDNA基因序列分析表明,菌株13-1与已报道的抗生素溶杆菌Lysobacter antibioticus DSM 2044(=ATCC29479)同源性高达99%。二者在所建系统发育树中处于同一分枝,将13-1菌株鉴定为抗生素溶杆菌(L.antibioticus)。Strain 13-1 was gram negative biocontrol bacterial strains isolated from Amorphophallus konjac. In vitro antagonistic assay showed that strain could suppress the growth of seven plant bacterial pathogens and eight pant fungal pathogens. The results of phenotypic and general physiological and biochemical properties showed that strain 13-1 homologized with which descripted for genus Lysobcter. 16S rDNA determination and analysis was used for further identification, which showed that 16S rDNA sequence of strain 13-1 shared 99% homologies with published sequence of L.antibioticus DSM2044 from GenBank, and both sequences constituted a branch in phylogenetic tree. Based on these results, it is considered that the strain 13-1 belongs to one strain of L.antibioticus.

关 键 词：Biolog鉴定 16S rDNA测序 抗生素溶杆菌(L.antibioticus) 生物学特性 

分 类 号：S476.1[农业科学—农业昆虫与害虫防治]