海洋环境中难培养微生物的寡营养培养  

Culture Methods of the Oligotrophic Marine Microbes

作　　者：田甜[1,2,3] 李冬梅[2,3] 戴世鲲[1,3] 殷克东[2] 孙慧敏[1,3] 李翔 

机构地区：[1]中科院南海海洋研究所中科院海洋生物可持续利用重点实验室广东省海洋药物重点实验室,广东广州510301 [2]中国科学院南海海洋研究所热带海洋环境动力学实验室,广东广州510301 [3]中国科学院研究生院,北京100049

出　　处：《微生物学通报》2009年第7期1031-1039,共9页Microbiology China

基　　金：国家基金委基金项目(No.40676074);大洋协会项目(No.DYXM115-02)研究的一部分

摘　　要：海洋中存在着丰富的微生物资源,但迄今为止能够在实验室培养的微生物却不到1%,而且能够通过培养得到的环境优势种更少,这成为当代环境微生物学研究和海洋资源开发的最大障碍。过去十多年来,通过不断改进培养方法和检测手段,发明了许多新颖独特的技术,提高了培养效率。特别是通过海洋微生物的寡营养培养技术,分离并命名了一些难培养微生物,给予人们极大的启发。海洋微生物资源的可持续性开发和利用,是21世纪人类发展的重要方向,是我们研究海洋微观世界的基础,值得微生物学界同仁的共同关注。Molecular methods and fluoroscopic techniques suggest that rich microbial diversity exist in the marine environment, but less than 1% of these microbes can be cultured in the laboratory conditions, and that the cultivable dominant species were even less. This limitation has long been a barrier to the development of environmental microbiology and the utilization of marine resources. In the past decade, novel methods for culture and detection of these uncultured marine microbes have successfully applied to obtain several conventionally-uncultured microbes including those from extreme environments. Those progresses have inspired researchers greatly. Developments in the research of marine microbial resources are an important basis for the study of the micro-world and deserve increasing scientific attention.

关 键 词：难培养细菌 寡营养培养 海洋微生物 系统分类学 

分 类 号：Q93-3[生物学—微生物学]