水稻中一个与OsHSP90互作的新基因特性分析  

Characteristic Analysis on a Novel Gene Interacted with OsHSP90 from Rice

作　　者：马炳田[1,2] 万佳[3] 张琦[2] 江斌[1] 徐永菊[1] 李冬梅[1] 徐正君[1,2] 

机构地区：[1]四川农业大学水稻研究所,四川温江611130 [2]四川农业大学西南作物基因资源与遗传改良教育部重点实验室,四川雅安625014 [3]四川省农业厅植物检疫站,四川成都610041

出　　处：《中国水稻科学》2009年第4期342-348,共7页Chinese Journal of Rice Science

基　　金：教育部长江学者和创新团队发展计划资助项目(IRT0453);国家科技支撑计划资助项目(2007BAD81B00)

摘　　要：OsHSP90是水稻中一类可以在不同逆境条件下被诱导的基因。利用酵母双杂交系统,在水稻低温和干旱cDNA文库中筛选出1个与OsHSP90互作的未知基因,命名为OsHSPBP。序列分析表明,该基因全长1321 bp,编码一个具有244个氨基酸残基的多肽,推测分子量为25.5 kD。在OsHSPBP蛋白的97-132位氨基酸残基间存在tify功能域,185-211位氨基酸残基构成CCT_2基序,与其他植物中的同源蛋白相似性介于30.66%-70.40%。经分析,该基因启动子区域存在6种不同的逆境反应顺式作用元件。RT-PCR半定量结果表明,OsHSP90与OsHSPBP在逆境胁迫下,均能被不同程度地诱导并呈现相似的表达趋势,说明两者可能与水稻逆境胁迫的应答和信号传递有关。OsHSP90 is an abiotic stress-inducible gene in rice. From the chilling and drought-inducible cDNA library of rice, a novel gene, OsHSPBP, which interacted with OsHSPgO was identified by using yeast two-hybrid system. The 1321 bp full length cDNA of OsHSPBP was cloned. Its open reading frame （ORF） predicts a 244-amino acid（aa） polypeptide with calculated molecular weight of 25.5 kD. A tify motif and a CCT_2 motif were found at the aa 97-132 and aa 185--211, respectively. Sequence similarity analysis indicated that the OsHSPBP had 30.66 %- 70.40% identities with the tify family proteins from other plants. Among the putative promoter region, six factors matched to cis-elements related to the abiotic stress response were identified using the PlantCARE software. Semi-quantitative RT-PCR showed that the OsHSP90 and OsHSPBP might he detected in the similar degree under different stress-treatments, suggesting that OsHSP90 and OsHSPBP could be involved in the signal transmission and response to the abiotic stress in rice.

关 键 词：水稻 逆境胁迫 诱导基因 基因表达 氨基酸序列 酵母双杂交系统 

分 类 号：S511[农业科学—作物学]