熊胆对人组织细胞淋巴瘤细胞系U-937的分化诱导作用  

BEAR BILE-INDUCED DIFFERENTIATION OF HUMAN HISTIOCYTIC LYMPHOMA CELL LINE(U-937)IN VITRO

作　　者：李秀森[1] 任蕴芳[1] 田洁[1] 卢涌泉[2] 

机构地区：[1]军事医学科学院基础医学研究所 [2]军事医学科学院仪器测试中心

出　　处：《军事医学科学院院刊》1990年第1期36-40,共5页Bulletin of the Academy of Military Medical Sciences

摘　　要：本文利用细胞化学、形态学、功能试验及单克隆抗体间接免疫荧光测定细胞表面抗原的方法,观察了熊胆对人组织细胞淋巴瘤细胞系 U-937的分化诱导作用。经400μg/ml 浓度的熊胆处理6天后,约有60%以上的 U-937细胞分化为形态和功能上成熟的单核-巨噬样细胞,细胞吞噬能力及分泌溶菌酶水平升高,细胞表面抗原有所变化。但熊胆对 U-937细胞增殖的抑制作用不明显。小剂量维生素 A 酸(RA)不加强熊胆对 U—937的分化诱导作用。This paper reports the induction of differentiation of U-937 cell line(monoblast)by bear bile.The cells were cultured at initial concentration 1.6-2×10~5/ml in RPMI-1640 medium with bear bile(400μg/ml)and incubated in 5% CO_2 at 37℃.On the sixth day, more than 60% of U-937 cells were induced to differentiate into monocyte-macrophage-like cells which was confirmed with the morphology,phagocytosis,lysozyme secretion,α-NAE staining and the change of surface membrane antigens in our experiments.But the growth of the U-937 cells was not inhibited by bear bile and low dose RA did not potentia- te the bear bile induced differentiation of U-937 cells.

关 键 词：人组织细胞 淋巴瘤细胞 熊胆 

分 类 号：R979.19[医药卫生—药品]