生长抑素对人脐血CD34+细胞增殖能力的影响  

Effects of somatostatin on proliferation ability of human CD34+ cells derived from umbilical cord blood*

作　　者：王利[1] 王春晖[2] 黄明慧[2] 唐承薇[2] 

机构地区：[1]重庆医科大学附属第一医院血液科,400016 [2]四川大学华西医院人类疾病生物治疗教育部重点实验室人类疾病相关多肽研究室,成都610041

出　　处：《重庆医学》2009年第14期1728-1729,1731,共3页Chongqing medicine

基　　金：国家杰出青年基金资助项目(39725012)。

摘　　要：目的探索生长抑素（SST）对造血干／祖细胞增殖能力的影响以及可能机制。方法采用免疫磁殊分选技术纯化人脐血CD34^＋细胞；SST孵育人CD34^＋细胞后，体外集落形成、扩增培养了解细胞增殖；ELISA测定细胞内外TNF-α、TGF-β水平；RT—PCR分析其受体亚型。结果在1×10^-6～1×10^-10mol／L浓度范围内，SST抑制人CD34^＋细胞集落形成及扩增倍数，其细胞集落形成抑制与SST浓度呈正相关（r=0．903，P〈0．01）。SST使CD34^＋细胞内、外TNF-α及TGF-β1浓度显著增加（P〈0．05）；人脐血CD34^＋细胞表达SST-3型受体。结论SST通过人脐血CD34^＋细胞上SST-3型受体介导，提高造血抑制因子TNF-α、TGF-β水平，抑制人CD34^＋细胞的增殖、维持其干细胞特性。Objective To investigate the effects and mechanisms of somatostatin （SST） on proliferation ability of hematopoietie/ progenitor stem ceils （HSC）. Methods Mononuclear cell （MNC） derived from human cord blood was isolated by Ficoll, then MACS assay was used to purify human CD34^＋ cells from MNC; After treated with SST, colony forming unit （CFU） enumeration and amplification were clone to assess the effects on proliferation of human CD34^＋ cells;TNF-α and TGF-β in human CD34^＋ cells or its supernatant were measured with ELISA;reverse transcriptation polymerase chain reaction was used to quantify receptor for SST （SSTR） and detected its subtype. Results SST in concentration of1×10^-6～1×10^-10mol/L inhibite proliferation of human CD34^＋ cells. The inhibition of CFU forming bore a positive relation with the concentration of SST（r=0. 903,P〈0.01）. The concentrations of TNF-α or TGF-β1 in human CD34^＋ cells treated with SST were increased when compared to control group（P〈 0.05）. Human CD34^＋ cells expressed receptor for SST-3. Conclusion Mediated by SSTR-3 in Human CD34^＋ cells,SST inhibite proliferation of Human CD34^＋ cells due to increasing the intra cell concentrations of TNF-α and TGF-β, the inhibitive cytokines for hematopoiesis. SST tends to keep characteristics of Human CD34^＋ cells and inhibit the differentiation of Human CD34^＋ cells to granuloeyte-monocytes.

关 键 词：造血干细胞 生长抑素 造血抑制 

分 类 号：R341[医药卫生—基础医学]