昆布多糖对大鼠骨髓巨噬细胞生物活性的影响  

Effect of laminarin on bioactivity of bone marrow macrophage*

作　　者：肖青[1] 董浦江[1] 王利[1] 张红宾[1] 杨泽松[1] 

机构地区：[1]重庆医科大学附属第一医院血液科,400016

出　　处：《重庆医学》2009年第14期1730-1731,共2页Chongqing medicine

基　　金：重庆市卫生局科学基金资助项目(07-2-036)。

摘　　要：目的研究昆布多糖对大鼠骨髓巨噬细胞生物活性的影响。方法制备并体外培养大鼠骨髓造血干/祖细胞、巨噬细胞;制备昆布多糖作用后的骨髓巨噬细胞条件培养液,测定培养液中造血生长因子促红细胞生成素(EPO)、粒-巨噬细胞集落刺激因子(GM-CSF)、白细胞介素-3(IL-3)表达及活性;测定昆布多糖条件培养液作用后造血祖细胞的集落产率。结果经昆布多糖诱导的骨髓巨噬细胞培养上清液可显著提高骨髓造血祖细胞的集落产率;经昆布多糖诱导后,骨髓巨噬细胞的EPO、GM-CSF和IL-3的表达增高。结论昆布多糖可刺激骨髓巨噬细胞造血因子表达增高,从而促进髓系多向性造血祖细胞(CFU-Mix)、红系祖细胞(CFU-E)、粒-单系造血祖细胞(CFU-GM)的增殖分化。Objective To investigate the effect of laminarin on bioactivity of bone marrow macrophage. Methods The techniques of hematopoietic stem/progenitor cell culture and bone marrow macrophage culture in vitro, biological assay of hematopoietic growth factor in culture media of bone marrow macrophage and western blot were used. Results The culture supernatant of BMФ induced by laminarin could enhance the number of CFU-Mix,CFU-E,CFU-GM;the expression of erythropoietin, GM-CSF, IL-3 in BMФinduced by laminarin was much stronger than that in the control group. Conclusion Laminarin can increase the expression of hematopoietic cytokines synthesized by macrophage, EPO,GM-CSF and IL-3, which can consequently promote the proliferation and differentiation of CFU-Mix,CFU-E and CFU-GM.

关 键 词：昆布多糖 骨髓巨噬细胞 生物活性 

分 类 号：R285.5[医药卫生—中药学]