基于纳米金增强吸附伏安分析的电化学免疫传感技术用于蛋白分子检测  

Nano-based payments adsorptive voltammetric strengthen the analysis of the immune electrochemical sensing technology for detection of protein molecules

作　　者：王潇蕤[1,2] 周敬良[1] 张艳丽[1] 蒋健晖[1] 

机构地区：[1]化学生物传感与计量学国家重点实验室,湖南大学化学化工学院,湖南长沙410082 [2]湖南化工职业技术学院应用化学系,湖南株洲412004

出　　处：《化学传感器》2009年第2期18-24,共7页Chemical Sensors

摘　　要：基于纳米金标记和金增强表面吸附伏安分析的新型电化学免疫传感器。以人免疫球蛋白G(hIgG)为模型分析物,使固定化抗体与分析目标物hIgG及纳米金标记的hIgG抗体结合于电极表面,通过金沉积使电极表面的纳米金颗粒直径增大。由于金增强时间在几分钟内就可完成,和传统的银增强相比,缩短了增强时间,加快了分析速度。研究结果表明,该法灵敏度高,选择性好,重现性好,操作简便,可望成为一种有潜力的新型高灵敏度免疫传感技术。Based on nano-gold mark and the enhanced surface analysis of a new type of electrochemical voltage immunosensor. Human immunoglobulin G （h IgG） model for the analysis of the material so that the immobilized antibodies and analysis of the target h IgG and the gold nano-h IgG antibody binding to the electrode surface, so that the gold electrodes deposited on the surface of gold nano-particles in diameter by Great. As the time to enhance the gold will be completed in a matter of minutes, and strengthen the traditional silver, shortened the time to strengthen and speed up the analysis of speed. The results show that the law of the high sensitivity and selectivity, reproducibility, and easy to operate, is expected to have the potential to become a new type of highly sensitive immune sensing technology.

关 键 词：纳米金 生物分子固定 电化学免疫传感器 

分 类 号：TP29-AI[自动化与计算机技术—检测技术与自动化装置]