MeJA、ABA与BA对黄瓜离体子叶叶绿素合成的影响  

Effects of MeJA,ABA and BA on Chlorophyll Synthesis in Excised Cotyledons of Cucumis sativus

作　　者：甘国平 

机构地区：[1]湖北省荆门市农业科学研究所,湖北荆门448000

出　　处：《江西农业学报》2009年第7期71-74,共4页Acta Agriculturae Jiangxi

摘　　要：将光照一段时间后的黄瓜离体子叶置于黑暗条件下,外源茉莉酮酸甲酯(MeJA)对黄瓜离体子叶叶绿素积累的抑制效果比光照条件下脱落酸(ABA)对黄瓜离体子叶叶绿素积累的抑制效果要强烈,苄基腺嘌呤(BA)与等摩尔浓度的MeJA或ABA混合使用可以完全中和它们的抑制作用。与ABA相反,MeJA可以增加在黑暗下预培养的子叶叶绿素合成的抑制作用,而ABA的抑制效果没有改变;经测定绿色子叶中的氨基乙酰丙酸含量发现,植物激素(BA)与叶绿素含量和ALA的积累密切相关。对于氨基乙酰丙酸合成,MeJA的抑制效果也强于ABA。本研究结果表明:MeJA和ABA两种抑制剂在叶绿素合成中有不同的抑制机制。MeJA优先抑制初期在黑暗条件下叶绿素的积累,而ABA的抑制性更突出表现在光照下叶绿素合成反应中。Except for the first stage of illumination, exogenous methyl ester of jasmonic acid （MeJA） had much stronger inhibitory effect than abscisic acid （ABA） on the accumulation of chlorophyll in excised cotyledons of Cucumis sativus exposed to the controlled light conditions. Benzyladenine （BA） applied in a combination with MeJA or ABA at equimolar concentration could completely neu- tralize their inhibitory effect. In contrast to ABA, preincubation of cotyledons with MeJA in the dark could increase its inhibitory effect on chlorophyll synthesis, while the inhibitory effect of ABA remained almost unchangeable. Determination of aminolevulinic acid （ALA） in green cotyledons revealed a close correlation between the effect of BA on chlorophyll content and ALA accumulation. MeJA inhibited the synthesis of ALA to a greater extent than ABA. Our results suggested that MeJA and ABA had different mechanisms of in- hibition on chlorophyll synthesis： MeJA preferentially inhibited chlorophyll accumulation at the level of chlorophyll precursors in the dark, while ABA exerted its effect on the light - dependent reaction of chlorophyll synthesis.

关 键 词：脱落酸 茉莉酸甲酯 苄基腺嘌呤 叶绿素含量 氨基乙酰丙酸 黄瓜 离体子叶 

分 类 号：S642.2[农业科学—蔬菜学]