金属组学:Wood-Ljungdahl通路中的金属蛋白/金属酶  

Metalloproteins/metalloenzymes for the synthesis of acetyl-CoA in the Wood-ljungdahl pathway

作　　者：朱小飞[1] 谭相石[1,2] 

机构地区：[1]复旦大学化学系,上海200433 [2]复旦大学生物医学研究院,上海200433

出　　处：《中国科学（B辑）》2009年第7期607-619,共13页Science in China(Series B)

基　　金：国家自然科学基金(批准号:20771029);上海市重点学科建设项目(批准号:B108);上海市浦江计划项目(批准号:08PJ14017)资助

摘　　要：本文概述了厌氧微生物的Wood-Ljungdahl通路及通路中的一组金属蛋白/金属酶,主要介绍该通路的来源、过程及通路中的四种金属蛋白/金属酶:甲酸脱氢酶、钴铁硫蛋白、乙酰辅酶A合成酶和CO脱氢酶.甲酸脱氢酶催化CO2和甲酸的可逆氧化还原,是CO2转化为甲酸进而转化为甲基四氢叶酸的关键金属酶;钴铁硫蛋白是该通路中的甲基转换器,接受甲基四氢叶酸的甲基之后再传递给乙酰辅酶A合成酶;CO脱氢酶催化CO2与CO之间的可逆氧化还原;乙酰辅酶A合成酶通过浓缩甲基、CO和辅酶A而催化乙酰辅酶A的合成.本文重点对这四种金属蛋白/金属酶的结构、性质、功能及催化机理的研究进展进行了综述.This paper focuses on the group of metalloproteins/metalloenzymes in the acetyl-coenzyme A synthesis pathway of anaerobic microbes called Wood-Ljungdahl pathway, including formate dehydrogenase （FDH）, corrinoid iron sulfur protein （CoFeSP）, acetyl-CoA synthase （ACS） and CO dehydrogenase （CODH）. FDH, a key metalloenzyme involved in the conversion of carbon dioxide to methyltetrahydrofolate, catalyzes the reversible oxidation of formate to carbon dioxide. CoFeSP, as a methyl group transformer, accepts methyl group from CH3-Hafolate and then transfers it to ACS. CODH reversibly catalyzes the reduction of CO2 to CO and ACS functions for acetyl-coenzyme A synthesis through condensation of methyl group, CO and coenzyme A, to finish the whole pathway. This paper emphasizes the structure, function and reaction mechanisms of these enzymes.

关 键 词：Wood—Ljungdahl通路 甲酸脱氢酶 钴铁硫蛋白 乙酰辅酶A合成酶 CO脱氢酶 

分 类 号：Q55[生物学—生物化学]