不同立克次体DNA的G+Cmol%含量分析  

COMPARISON OF DNA G+C MOL% OF RICKETTSIAE ISOLATED FROM SOME REGIONS IN CHINA

作　　者：陈香蕊[1] 陈万荣[1] 陈建龙 胥照平[1] 郭兆彪[1] 汪民[1] 

机构地区：[1]军事医学科学院微生物流行病研究所

出　　处：《军事医学科学院院刊》1990年第3期185-187,共3页Bulletin of the Academy of Military Medical Sciences

基　　金：国家自然科学基金资助课题

摘　　要：本文以普氏斑疹伤寒立克次体和斑点热立克次体为参照,对我国斑点热立克次体分离株的代表株 DNA 的 G+C mol%含量进行了分析比较,结果指出,斑疹伤寒群与斑点热群立克次体的 G+C mol%含量不同,分别为29.1和32.05;国内分离的精河株和内-01株分别为32.7和32.4,与参照的斑点热立克次体 Barbash 株(32.5)基本一致,054株的 G+C mol%是30.6,低于斑点热群的 G+C mol%数值,高于斑疹伤寒群的含量,揭示了该株 DNA 的 G+C 含量的特殊性,这是否意味着054株的特殊分类地位或是否是新种有待深入研究。The DNA G+C content (moles percent,G+C mol%) of three strains of spotted fe- ver group (SFG) of rickettsiae (SFGR) isolated from some regions in China were analyzed by the method of determination of thermal denaturation temperature by UV-spectrometry.E. coli and R.prowazekii were used as standard references.The results revealed that DNA base composition was different in the typhus and SFGR and the average value of SFGR was 32.05.The value of Jinghe,Heilongjiang 054 and Innermongolia 01 strains were32.7, 30.6 and 32.4 respectively.It is interesting that the value of 054 was different from other members of SFGR and R.prowazekii.

关 键 词：立克次体 DNA 含量 

分 类 号：R376.1[医药卫生—病原生物学]