蛹虫草无性型菌丝体提取液体外抗氧化活性研究  

Evaluation of the antioxidant activity of extracts from anamorphic mycelia of Cordyceps militaris in vitro

作　　者：吕国英[1] 潘慧娟[1] 吴永志[1] 范雷法[1] 

机构地区：[1]浙江省农业科学院园艺研究所,杭州310021

出　　处：《菌物学报》2009年第4期597-602,共6页Mycosystema

摘　　要：分别测定了蛹虫草无性型菌丝体不同溶剂提取液在不同抗氧化模型中的抗氧化作用。结果表明,各种提取液对二苯基苦味酰基苯肼自由基(·DPPH)和羟自由基(·OH)均有显著的清除作用:在50mg/mL时,去离子水、70%乙醇和70%丙酮提取物对DPPH自由基的清除率分别为89.2%、83.6%和75.9%;70%乙醇提取物在30mg/mL时对·OH自由基的清除率达到100%;在50mg/mL时70%丙酮和去离子水提取物的·OH自由基清除率分别为95.6%和89.6%。在一定浓度下,各提取液对邻苯三酚自氧化也均有抑制作用;不同溶剂提取物的还原能力强弱为:70%乙醇>去离子水>70%丙酮。各种提取物的抗氧化组分不同,提取物浓度与抗氧化性成正相关。The extracts of different solvents from anamorphic mycelia of Cordyceps militaris were analyzed for their antioxidant activities in different test systems. The results showed that all kinds of extracts have evident effects on scavenging 1,1-diphenyl-2-picrylhydrazyl radical （-DPPH） and hydroxyl radical. For the extracts of deionized water, 70% ethanol and 70% acetone at 50mg/mL, DPPH radical scavenging activity was 89.2%, 83.6% and 75.9%, respectively. When the concentration of 70% ethanol extracts was at 30mg/mL, hydroxyl radical scavenging activities was up to 100%. When the extracts of 70% acetone and deionized water were at 50mg/mL, hydroxyl radical scavenging activities was 95.6% and 89.6%, respectively. In certain concentration, the extracts contain restraining effects on pyrogallol autooxidation. Reducing power of the extracts followed in the order： 70% ethanol 〉deionized water 〉70% acetone. The concentration of the extracts is in direct proportion to the antioxidant activity, and the antioxidant components may be different in various extracts.

关 键 词：二苯基苦味酰基苯肼 羟自由基 邻苯三酚自氧化 还原能力 

分 类 号：S567.35[农业科学—中草药栽培]