晚期糖基化终产物及其受体在白细胞中表达与老年人精神障碍的关系  

The correlation of serum level of advanced glycation end products and the expression of receptor for advanced glycation end products in white blood cells with senile mental disorder

作　　者：黎雪松[1] 王明[2] 刘誉[2] 

机构地区：[1]佛山市第三人民医院老年科,528041 [2]广州暨南大学医学院生化教研室

出　　处：《中华老年医学杂志》2009年第7期587-589,共3页Chinese Journal of Geriatrics

摘　　要：目的探讨血液中晚期糖基化终产物（AGEs）及其受体（RAGE）表达水平与老年人精神障碍性疾病的关系。方法将观察对象分为健康对照组31例、阿尔茨海默病（AD）组36例、血管性痴呆组20例、脑血管病所致精神障碍组28例。以荧光分光光度法测定各组血清AGEs水平，以逆转录多聚酶链式反应测定RAGE mRNA水平。结果血清AGEs水平在AD组、血管性痴呆组和精神障碍组分别为（477．1±36．2）AU／ml、（512．6±33．2）AU／ml和（415．2±32．5）AU／ml，均明显高于健康对照组（357．4±28．2）AU／ml（F=3．77，P〈0．05）。RAGE mRNA水平（RAGE／b—actin）分别为1．12±0．34、1．27±0．41和1．18±0．42，亦高于健康对照组的0．92±0．37（F=4．07，P〈0．01），但各疾病组间差异无统计学意义（F=0．979，P〉0．05）。白细胞中RAGE mRNA水平与血清AGEs呈正相关关系（r=0．442，P〈0．01）。结论AD、血管性痴呆、脑血管病所致精神障碍患者血中的AGEs及其受体RAGE mRNA水平均较同龄健康老年人明显升高，AGEs与RAGE的相互作用可能直接或间接参与了老年人精神障碍性疾病的病理变化。Objective To investigate the correlation of serum advanced glycation end products （AGEs） and the expression of receptor for AGE （RAGE） with mental disorders in senile people. Methods All the subjects aged 65-67 years were divided into 4 groups., healthy control （31 cases）, Alzheimer＇s disease （AD） （36 cases）, vascular dementia （VD） （20 cases） and Rental disorder （MD） （28 cases）. Fluorospectrophotometry was carried out to measure the AGEs levels in serum samples, and reverse transcription polymerase chain reaction （RT-PCR） was used to determine the mRNA levels of RAGE in white blood cells. Results AGEs levels were 477.1± 36.2, 512.6 ± 33.2 and 415.2±32.5 AU/ml in AD, VD and MD group, respectively, which were higher than that in healthy control group （357.4±28.2） AU/ml （F=3.77,P〈0.05）. The mRNA levels of RAGE（RAGE/bactin） were found to be 1.12±0.34, 1.27±0.41 and 1.18±0.42 in AD, VD and MD group, respectively, which were also significantly higher than that in healthy control group（0. 92±0. 37, F= 4.07,P〈0.01）. However, no significant differences were found in RAGE mRNA levels among the three groups of AD,VD and MD （F=0. 979,P〉0.05）. The mRNA levels of RAGE in WBCs were found to be positively correlated with the serum AGEs levels （r=0. 442, P〈0.01）. Conclusions Compared with patients in healthy control group, the serum AGEs levels and RAGE mRNA levels are significantly increased in patients with AD, VD and MD. The interaction between AGEs and RAGE may be involved in the pathogenesis of mental disorders in senile people.

关 键 词：糖基化终产物 高级 阿尔茨海默病 痴呆 血管性 精神障碍 

分 类 号：R686[医药卫生—骨科学]