豚鼠光老化模型的建立和氮氧化物Tempol防护作用的研究  

Study on the establishment of skin photoaging model in guinea pig and on the protective effects of nitroxide tempol

作　　者：严淑贤[1] 陈国梁[1] 胡跃[1] 徐昱[1] 孙新芬[1] 廖康煌[1] 

机构地区：[1]复旦大学附属华山医院皮肤科 复旦大学上海医学院皮肤性病学系,上海市200040

出　　处：《中华老年医学杂志》2009年第7期593-596,共4页Chinese Journal of Geriatrics

摘　　要：目的探讨建立豚鼠皮肤的光老化模型及外用氮氧化物Tempol对豚鼠光老化皮肤的保护作用。方法以模拟日光光源（SSR）建立豚鼠皮肤的光老化模型，以苏木素一伊红和Weigert染色法及透视电镜观察真皮结构、成纤维细胞和弹力纤维结构的变化。豚鼠在每次照射前外用5mg／ml或0．5mg／ml的Tempol，探讨Tempol对上述变化的保护作用。结果豚鼠以亚红斑量SSR照射17周，光照部位真皮浅层出现了典型的光化性弹力纤维变性损害，成纤维细胞损伤甚至破碎。照射前外用Tempol可以预防上述表现出现，Tempol以5mg／ml保护作用更强。结论利用豚鼠可构建稳定的光老化动物模型，抗氧化剂Tempol对豚鼠皮肤光老化损害有防护作用。Objective To explore the establishment of skin photoaging model and the protective effects of nitroxide tempol on skin in guinea pig. Methods The guinea pig skin photoageing model was established by using solar-simulaten radiation （SSR）. Dermal structure was observed with hematoxylin-eosin staining. The structure and expression of elastic fiber were analyzed by Weigert＇s staining. The ultrastructure of dermal fibroblasts and elastic fiber were observed by electron microscopy. Tempo/ was used before each exposure at the concentration of 5 mg/ml or 0. 5 mg/ml, and the protective effects of tempol on skin were assessed. Results After seventeen weeks＂ exposure, there was typical ＂solar elastosis＂ damage in the upper dermis. Mature elastic fibers were severely degraded and there was large amount of elastotic material accumulated in the upper dermis. Dermal fibroblasts appeared metabolically hyperactive and mitochondria in the cells were damaged. Some cells even broke up. Tempol at the concentration of 5 mg/ml or 0.5 mg/ml could prevent photodamage of the photoageing model in the dermis, and Tempol at the concentration of 5 mg/ml had stronger protective effects. Conclusions Guinea pig can be applied as an useful animal model of skin photoageing. Antioxidant tempol has photoprotective effects on pbotodamage of the photoageing model in guinea pig and can be used as an anti-photoageing agent.

关 键 词：紫外线 皮肤衰老 抗氧化剂 环氮氧化物 

分 类 号：R686[医药卫生—骨科学]