鸡肠道抗菌肽的提取及活性研究  

The Preparation and Activity of an Antimicrobial Peptide from Chicken Intestine

作　　者：谭淑樱[1] 王海英[1] 祁克宗[1] 彭开松[1] 

机构地区：[1]安徽农业大学动物科技学院,合肥230036

出　　处：《家禽科学》2009年第7期3-6,共4页Poultry Science

基　　金：国家"863"计划(2006AA10Z320);安徽省优秀青年基金(04041042)

摘　　要：取新鲜鸡肠黏膜超声波破碎,乙酸浸提,经Sephadex G-50层析后,收集具有抑菌活性的组分进行超滤离心,抑菌实验表明滤过物具有抑菌活性。滤过物经SDS-PAGE分析为一个条带,分子量约为6.078KD,得到电泳纯的鸡肠道抗微生物肽。该肽具有一定的耐热性,但经90℃以上热处理后活性下降较大;pH值对其活性影响较大,在pH6～8时活性最强。After the intestinal mucous membranes of chicken were treated by ultrasonic treatment and acid extraction,an antimicrobial peptide was isolated from the intestine of chicken by Sephadex G-50 gel filtration chromatography and centrifugal uhrafiltration.The filtration material was analyzed by SDS-PAGE and the result showed that an antimicrobial peptide was purified and its molecule weight was about 6.078kD.The antimicrobial peptide was heat-resistant, but its activity descended obviously after heated at 90℃.The activity of the antimicrobial peptide was affected by pH and it was the strongest at pH6-8.

关 键 词：鸡 肠黏膜 抗微生物肽 温度 PH值 

分 类 号：S831.1[农业科学—畜牧学]