尼尔雌醇联合左炔诺孕酮对人成骨肉瘤MG-63细胞株雌激素受体亚型表达作用  

Comparison of the effects of NYL and/or LNG on the expression of ER subtypes in human osteosarcoma MG-63 cell lines

作　　者：杨开初 廖二元[2] 罗湘杭 周后德 

机构地区：[1]武警广东边防总队深圳医院内科,深圳518029 [2]中南大学湘雅二医院内分泌所

出　　处：《中国骨质疏松杂志》2009年第7期538-542,512,共6页Chinese Journal of Osteoporosis

摘　　要：目的观察NYL（尼尔雌醇）联合LNG（左炔诺孕酮）干预培养人成骨肉瘤MG-63细胞株在雌激素受体（ER）亚型mRNA表达过程中是否存在正性协同作用。方法用直接原位RT-PCR及激光共聚焦显微镜观察不同药物干预组的MG-63细胞株ERα、ERβmRNA表达。结果NYL及LNG两种药物联合干预培养组的ERα或ERβ的荧光强度均明显超过两种药物单独干预培养组。结论NYL和LNG在诱导MG-63细胞株ER亚型表达上调过程中具有正性协同作用,LNG可具有独立预防骨质疏松作用。Objective To determine whether positive synergistic effect is present on inducing the up-regulation of mRNA expression of ER subtypes by combined intervention with NYL and LNG. Methods Direct In situ RT-PCR combined with LSCM was applied to observe the mRNA expression of ERa and ERβ in MG-63 cell lines which were cultured with NYL and/or LNG intervention. Results The fluorescence intensity of both ER subtypes on the slides which were processed with combined intervention MG-63 cells are much more intense than that of ER subtypes on the slides which were processed with alone intervention. Conclusion Combined intervention with NYL and LNG has positive synergistic effects on the up-regulation of mRNA expression of ER subtypes in MG-63 cell lines. LNG maybe have the effects of preventing postmenopausal women suffering bone loss.

关 键 词：尼尔雌醇 左炔诺孕酮 雌激素受体亚型 原位RT-PCR 激光共聚焦显微镜 

分 类 号：R592[医药卫生—老年医学]