灯盏花素在多壁碳纳米管修饰玻碳电极上的电化学行为及其测定  

Electrochemical behavior and determination of breviscapine at multi-wall carbon nanotubes modified glassy carbon electrode

作　　者：郭英[1,2] 贝玉祥[1] 高云涛[1] 张文刚 

机构地区：[1]云南民族大学化学与生物技术学院,昆明650031 [2]郧阳师范高等专科学校化学系,丹江口442700 [3]云南白药集团股份有限公司昆明650118

出　　处：《药物分析杂志》2009年第7期1130-1133,共4页Chinese Journal of Pharmaceutical Analysis

基　　金：云南省社会发展科技计划项目(2007B148M)

摘　　要：目的:制备多壁碳纳米管(MWNT)修饰玻碳电极,并研究灯盏花素在该电极上的电化学行为及其测定方法。方法:循环伏安法和差示脉冲伏安法。结果:与裸玻碳电极(GCE)相比,MWNT膜修饰电极能显著提高灯盏花素的氧化峰电流。在pH=7.0的磷酸缓冲溶液中,灯盏花素在MWNT/GCE电极上出现1组氧化还原峰,Epa=0.19V,Epc=0.07V。灯盏花素氧化峰电流与其浓度在2×10-7～6×10-5mol·L-1范围内呈线性关系,检出限为2×10-7mol·L-1。灯盏花素样品进行5次平行测定的RSD为0.37%,平均回收率为99.5%。结论:MWNT膜对灯盏花素的电化学氧化有明显的催化作用,本法是一种可靠、快捷、灵敏的检测方法,可以用于灯盏花素含量的测定。Objective： To fabricate multi -wall carbon nanotubes modified glassy carbon electrode （MWNT/ GCE） , the electrochemical behavior and determination of breviscapine on the electrode were investigated. Methods： Cyclic vohammetry and differential pulse voltammetry. Results ： The oxidation peak current of breviscapine inereased remarkably at the MWNT/GCE in contrast to that at the bare GCE. One pair of redox peaks of breviscapine at MWC- NT/GCE was obtained by cyclic vohammetry in phosphatic buffer solution （pH = 7.0） with 0. 19 V of Epa and 0.07 V of Epc. The oxidiztion peak is linearly related to the concentration of breviscapine in the range of 2×10^-7～6×10^-5mol·L^-1 and the detection limit reach of to 2 ×10^-7mol·L^-1. The RSD（n =5） was 0.37% for breviscapine. The average recovery was 97.5% - 103.1%. Conclusion： The MWMT/GCE exhibits eatalytic activity to the oxidation of breviscapine. The proposed method is accurate, sensitive and convenient for the determination of the conten of breviscapine.

关 键 词：灯盏花素 多壁碳纳米管 修饰电极 循环伏安法 

分 类 号：R917[医药卫生—药物分析学]