NaCl对三疣梭子蟹消化酶活力的影响  

The effects of NaCl on digestive enzyme activities of Portunus trituberculatus

作　　者：胡毅[1] 潘鲁青[1] 

机构地区：[1]中国海洋大学海水养殖教育部重点实验室,山东青岛266003

出　　处：《海洋科学》2009年第7期51-56,共6页Marine Sciences

基　　金：国家自然科学基金资助项目(30100140);天津市农业科技成果转化与推广资助项目(07050201)

摘　　要：作者采用酶学分析方法研究了反应介质中添加NaCl对三疣梭子蟹（Portunus tritubrculatus）中肠腺蛋白酶、胰蛋白酶、胰凝乳蛋白酶、羧肽酶A、羧肽酶B、淀粉酶活力的影响，反应介质中NaCl浓度设置为0、0．005、0．01、0．05、0．1、0．5、1．0和2．0mol／L8个梯度。实验结果表明，反应介质中添加NaCl对消化酶活力影响显著，除羧肽酶B外，添加NaCl对三疣梭子蟹消化酶均有激活作用，且所有消化酶都有很高的耐盐性。当反应介质中NaCl浓度为0．1～0．5mol／L时蛋白酶活力最大；0．5mol／L时胰蛋白酶活力最大；胰凝乳蛋白酶在反应介质中NaCl浓度为1mol／L时酶活力最大；羧肽酶A在反应介质中NaCl浓度为0．05-0．1mol／L时酶活力最大；NaCl对羧肽酶B有抑制作用，在反应介质中NaCl浓度为2mol／L的条件下，只有未添加NaCl时酶活力的60％；NaCl在0～2mol／L时对淀粉酶均有激活作用，其最大激活能力在0．5mol／L左右。The effects of NaCl on the activities ofprotease, trypsin, chymotrypsin, carboxype ptidase A, carboxypeptidase B and amylase of Portunus trituberculatus were studied by adjusting the assay mixture （0, 0.005, 0.001, 0.05, 0.1, 0.5, 1.0 and 2.0 mol/L） through using enzyme analytical method. The experimental results indicated that all enzymes were activated by low NaCl concentration except for carboxypeptidase B. Within the scope of these experimental concentrations, protease has higher activities at NaCl concentration around 0.1-0.5 mol/L, trypsin has the higher activity at NaCl concentration around 0.5 mol/L, chymotrypsin has a more high activity at NaCl concentration around 1 mol/L, carboxypeptidase A has a more high activity at NaCl concentration around 0.05-0.1 mol/L, carboxypeptidase B was inhibited by NaCl, and has 60% maximum activities at 2 mol/L NaCl. NaCl activated amylase within the scope of this experimental concentrations, amylase has the highest activity at NaCl concentration around 0.5 mol/L.

关 键 词：三疣梭子蟹(Portunus tritubrculatus) 消化酶 NACL 

分 类 号：Q175[生物学—水生生物学]