去势大鼠骨质疏松与股骨甲状旁腺激素受体1相关性研究  

The Correlation between castrate osteoporosis and femur parath yroid hormone receptor 1 in rats

作　　者：张松[1] 王四旺[1] 王超[1] 王伟华[2] 谢艳华[1] 

机构地区：[1]第四军医大学药学系药物研究所,西安710032 [2]第四军医大学生物技术中心,西安710032

出　　处：《陕西医学杂志》2009年第7期771-773,777,共4页Shaanxi Medical Journal

基　　金：国家自然科学基金(50577067)

摘　　要：目的:观测去势大鼠骨质疏松时血清雌二醇(E2)水平与甲状旁腺激素受体1(PTHR1)表达的关系。方法:雌性大鼠随机分为去势和假去势(即对照组)两组,大鼠切除双侧卵巢诱发骨质疏松症。术后3个月腹主静脉取血,测定各组大鼠血清E2水平;分别用小动物骨骼强度测定仪和双能X线骨密度仪检测右股骨生物学力学和骨密度;从左股骨中提取总RAN和总蛋白,分别检测PTHR1在转录和翻译水平上的变化。结果:去势大鼠血清E2含量为13.80±1.10pmol/L,对照组血清E2含量为23.51±1.20pmol/L;去势组右股骨生物学力度:折断力为9.89±0.65N/kg,压碎力为16.10±1.23N/kg,骨密度为0.0730±0.0075gms/cm2;对照组右股骨生物学力度:折断力为11.74±0.95N/kg,压碎力为19.38±1.84N/kg,骨密度为0.0839±0.0097gms/cm2。与对照组相比,去势大鼠PTHR1基因和蛋白水平表达量均明显降低。结论:去势不仅可引起大鼠E2水平明显降低,并诱发骨质疏松进而导致PTHR1基因和蛋白表达水平均显著下降,提示PTHR1表达下降可能与去势诱导大鼠骨质疏松密切相关。Objective： To explore the relationship between serum estradiol （E2） and expression of parathyroid hormone receptor 1 （PTHR1） in ovariectomized osteoporosis Rats. Methods： Twenty adult female rats were randomly divided into two groups ： the ovariectomized group and the model control group. The rats of castration group excised double ovaries and induced hormonoprivia. After three months, gained blood from cardinal vein and detected the level of serum E2; put all rats to death and got femur bone tissue with asepsis condition. Detected the transcriptional level of PTHR1 gene with RT-PCR. And detected the expression level of PTHR1 protein with Western blot. Results： In ovarieetomized groupthe level of serum E2 was 13. 80±1.10 pmol/L,the E2 of model control group was 23.51±1.20 pmol/L. In ovariectomized group right femur skeleton strength： fracture test was 9. 89±0. 65 N/kg, crushing test was 16.10±1.23 N/kg and bone density was 0. 0730±0. 0075 gms/cmz. In control group, right femur skeleton strength： fracture test was 11.74±0. 95 N/kg, crushing test was 19. 38 ± 1. 84N/kg and bone density was 0. 0839 ± 0. 0097gms/cm2. All indexes in ovariectomized group compared with model control group were obviously lower. The level of PTHR1 gene and protein in ovariectomized were group compared with model control were group obviously lower. Conclusions.. Castration could lower the level of serum E2 obviously and induce osteoporosis in rats. It is very interesting that the level of PTHR1 gene and protein are obviously lower. It implicated that PTHR1 might play an important role in ovariectomized rats to induce osteoporosis.

关 键 词：骨质疏松/免疫学 受体 甲状旁腺激素/免疫学 卵巢切除术 股骨 基因表达 大鼠 

分 类 号：R459.5[医药卫生—治疗学] R681[医药卫生—临床医学]