γ射线辐照外周血单个核细胞对肿瘤细胞杀伤作用的研究  

Studies on the killing effects of peripheral blood mononuclear cells irradiated by γ ray on human cancer cell

作　　者：白艳丽[1] 张献清[2] 夏爱军[2] 龚卫锋[1] 王兆龙[1] 李珊[1] 

机构地区：[1]西安市中心医院输血科,西安710003 [2]第四军医大学西京医院输血科

出　　处：《陕西医学杂志》2009年第7期786-789,共4页Shaanxi Medical Journal

基　　金：陕西省科学技术研究发展计划项目(2008k09-09)

摘　　要：目的:观察γ射线辐照外周血单个核细胞对培养人前列腺癌细胞系PC-3细胞的杀伤作用。方法:实验设PC-3P肿瘤细胞对照组、单纯外周血单个核细胞对照组、照射和未照射的外周血单个核细胞与肿瘤细胞共培养组,照射剂量为1Gy,吸收剂量率为17Gy/min。利用吖啶橙/溴化乙锭(AO/EB)荧光双染色法和MTT比色法,观察外周血单个核细胞对PC-3细胞的杀伤情况。结果:外周血单个核细胞在照射后体外培养至144h有大量细胞死亡,存活细胞明显少于未照射组,培养至240h时,照射与非照射组中存活的外周血单个核细胞均减少,但是在照射的各组中减少更为明显。在外周血单个核细胞与PC-3肿瘤细胞共培养时,照射组在培养至96h时,γ射线照射外周血单个核细胞对前列腺癌细胞的杀伤作用明显增强,杀伤活性明显增加。结论:γ射线辐照对某些外周血单个核细胞有损伤作用,但是γ射线辐照的外周血单个核细胞其杀伤肿瘤细胞活性增强。Objective： To observe the killing effects of peripheral blood mononuclear cells （PBMCs） irradiated by γ ray on cultured human prostate tumor cell line PC-3. Methods： The experiment cells were divided into PC-3 cell control group, PBMCs control group,PC-3 tumor cells with irradiated or non-irradiated PBMCs co-cultured groups. Radiation dosage was one gray, absorption dose rate was 17Gy/min , acridine orange/ethidium bromide （AO/EB） staining and MTT colorimetric assay were used to observe the killing effects of PBMCs on tumor cells in different period. Results： After culture for 144h , the dead PBMCs in irradiated group were much more than that in non-irradiated PBMCs group. In PBMCs and MKN-28 tumor cells co-cultured group, the killing effects of PBMCs on tumor cells in irradiation group was higher than that in nonradiation group from 96h to 240h. Conclusion：Gamma ray irradiation has damage effect on some PBMCs, but the killing effects of PBMCs irradiated by low dose radiation on tumor cells is increased.

关 键 词：前列腺肿瘤/辐射效应 单核细胞/辐射效应 Γ射线 

分 类 号：R512.62[医药卫生—内科学] R730.3[医药卫生—临床医学]