云南个旧及宣威地区肺癌p14^ARF和E2F-1水平的改变  

Expression of p14^ARF and E2F-1 in lung cancer in Gejiu and Xuanwei regions of Yunnan Province

作　　者：杨志鸿[1] 阮永华[2] 金克炜[2] 高倩[2] 张林[3] 

机构地区：[1]云南医学高等专科学校病理教研室,昆明650031 [2]昆明医学院病理教研室 [3]昆明医学院第一附属医院病理科

出　　处：《中华劳动卫生职业病杂志》2009年第7期411-415,共5页Chinese Journal of Industrial Hygiene and Occupational Diseases

基　　金：云南省教育厅科研基金项目（062066C）;云南省应用基础研究基金项目（2006C0042M）

摘　　要：目的探讨阅读框架基因p14^ARF和核转录因子E2F-1在云南个旧地区矿工肺癌，宣威地区农民肺癌组织中的表达及相互关系，寻找肺癌早期诊断特异性标记物及为治疗提供实验依据。方法采用免疫组化S-P法，检测30例个旧地区矿工肺癌和30例宣威地区农民肺癌及20例正常肺组织中p14^ARF和E2F-1蛋白的表达；采用原位分子杂交方法随机检测上述标本中25例个旧地区矿工肺癌、25例宣威地区肺癌及10例正常肺组织中E2F—1mRNA的表达。两种检测结果应用HPIAS-100计算机图文分析系统进行定量分析。结果个旧和宣威地区肺癌组织的p14^ARF蛋白表达明显低于正常肺组织，而E2F-1蛋白表达和mRNA表达均明显高于正常肺组织。p14^ARF和E2F-1蛋白表达的阳性单位在个旧肺癌组为16．44±4．85和47．39±5．43，宣威肺癌组为16．79±3．55和48．15±9．11，与正常肺组织组比较，差异均有统计学意义（P〈0．01）。个旧肺癌组E2F-1mRNA表达的阳性单位为48．58±7．75，宣威肺癌组为49．41±8．53，明显高于正常肺组织组，差异有统计学意义（P〈0．01）。在个旧、宣威肺癌组和正常肺组织组中，E2F—1mRNA和E2F-1蛋白表达的阳性单位呈正相关（r=0．833，P〈0．01），p14^ARF和E2F-1蛋白表达的阳性单位呈负相关（r=-0．830，P〈0．01）。结论个旧和宣威两地区肺癌组织中存在E2F-1基因的表达上调和p14^ARF基因的表达下调。Objective To investigate the correlations between p14^ARF and E2F-1, and the role of their alterations in the tumorigenesis of the lung cancer in Gejiu and Xuanwei regions in Yunnan Province for pro- viding the important experiment basis in revealing the molecular mechanism and looking for new markers for early diagnosis of lung cancer. Methods The expression of p14^ARF and E2F-1 was detected at theirs protein level by Immunohistoehemistry S-P method in 30 specimens of lung cancer of Gejiu tin miners,30 specimens of lung cancer of Xuanwei peasants and 20 specimens of normal lung tissue.E2F-1 mRNA was detected by ISH in 25 specimens of lung cancer of Gejiu tin miners,25 specimens of lung cancer of Xuanwei peasants and 10 specimens of normal lung tissue.The positive signals were quantitatively analysed by HPIAS-100. Results The positive unit （PU） of p14^ARF and E2F-1 was 16.44±4.85 and 47.39±5.43 in Gejiu group, and 16.79±3.55 and 48.15±9.11 in Xuanwei group. Expression of p14^ARF and E2F-1 protein in lung cancer of Gejiu and Xuanwei were statistically different compared with that in the normal lung （P〈0.01）respectively; The PU of E2F-1mRNA was 48.58±7.75 in Gejiu group, and 49.41±8.53 in Xuanwei group, which was higher than that in normal tissue group. The differences were significant（P〈0.01 ）. There was positive correlation between the expression of E2F-1 protein and E2F-1mRNA in Gejiu group, Xuanwei group and normal group （P〈0.01, r=0.833 ）. The expression of p14^ARF protein was significantly negatively correlated with the expression of E2F-1 protein （P〈0.01 ,r=-0.830）. Conclusion There is the over-expression of E2F-1 gene and the deletion of p14sat gene in the tumorigenesis of the lung cancer in Gejiu and Xuanwei regions in Yunnan Province. Over-expression of E2F-1 protein in lung cancer may be caused by enhanced transcription.

关 键 词：肺肿瘤 P14^ARF 转录因子 E2F-1 个旧 宣威 

分 类 号：R686[医药卫生—骨科学]